太子参醇提物的肠道益生及增强抗氧化作用

Intestinal Probiotic Function and Antioxidant Capacity-enhancing Effect of An Ethanolic Extract of Radix pseudostellariae

摘要:

本研究采用D-半乳糖模型鼠探讨太子参醇提物的体内抗氧化和肠道益生关系。选用72只ICR小鼠，分成6组，使用D-半乳糖致损的同时给予太子参醇提物干预，考察灌胃给予太子参醇提物对小鼠生长情况、抗氧化酶活和肠道功能的影响。结果表明经大孔树脂D101初步纯化的太子醇提物中含有皂苷类化合物613.48 mg/g干物质；与模型组相比，太子参醇提物高剂量组鼠肝脏SOD、CAT和GSH-PX酶活分别提高了16.64%、26.01%和20.30%，肾脏SOD、CAT和GSH-PX酶活分别提高了19.94%、23.37%和18.00%，脑部SOD、CAT和GSH-PX酶活分别提高了14.55%、13.06%和40.72%。通过提高肠道双歧杆菌、乳杆菌、拟杆菌和布劳特氏菌等有益菌属丰度，降低甲基杆菌属、理研菌属、普雷沃氏菌属等有害菌属丰度，降低肠道患病风险，恢复小肠绒毛结构，有效增强机体抗氧化水平。

Abstract:

In this study, D-galactose model mice were used to investigate the relationship between in vivo antioxidation and intestinal probiotic function of an ethanol extract of Radix pseudostellariae. Seventy-two ICR mice were selected and divided into 6 groups. D-galactose was used to induce damage, and a Pseudostellariae ethanol extract was used to intervene to investigate its effect on the growth, antioxidant enzyme activities and intestinal function of the mice. The results showed that the Radix pseudostellariae extract after purification by resin D101 contained saponins (613.48 mg/g dry weight). Compared with the model group, the activities of SOD, CAT and GSH-px of the liver of the HPEs group increased by 16.64%, 26.01% and 20.30%, respectively, with the activities of SOD, CAT and GSH-px of the kidney increasing by 19.94%, 23.37% and 18.00%, the activities of SOD, CAT and GSH-px of the brain increasing by 14.55%, 13.06% and 40.72%, respectively. By increasing the abundance of beneficial bacteria such as Bifidobacterium, Lactobacillus, Bacteroides and Blautiain the intestines, and decreasing the abundance of harmful bacteria such as Methylobacterium, Rikenella and Paraprevotella, the extract could reduce the risk of intestinal diseases, restore the structure of the small intestinalvilli and enhance effectively the body’s antioxidant level.