体外消化对藜蒿叶多酚含量及抗氧化活性的影响
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熊文(1996-),女,硕士,研究方向:农产品加工与贮藏工程 通讯作者:徐晓云(1970-),女,博士,教授,研究方向:农产品加工与贮藏工程

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湖北省农业科技创新中心农产品加工与综合利用团队(2019-620-000-001-31)


Effects of in Vitro Digestion on Phenolic Acid Content and Antioxidant Activity of Artemisia selengensis Turcz Leaves
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    摘要:

    本文研究了藜蒿叶提取物在模拟体外消化过程中酚酸含量、抗氧化活性以及对酪氨酸酶活力抑制率的变化。研究表明,经过模拟体外消化后藜蒿叶提取物中的绿原酸、1,5-双咖啡酰奎宁酸、3,4-双咖啡酰奎宁酸、3,5-双咖啡酰奎宁酸、4,5-双咖啡酰奎宁酸5种酚酸含量均显著性降低。总酚含量和DPPH、FRAP、ABTS抗氧化活性经过模拟体外消化后均显著性增加。消化后粗提物和纯化样总酚含量分别升高了73.57%、23.79%,DPPH抗氧化能力分别增强了36.17%、53.29%,FRAP抗氧化能力分别增强了28.71%、15.45%,ABTS清除自由基能力分别增强了163.67%和49.50%。此外,藜蒿叶粗提物对酪氨酸酶活力的抑制率在体外消化过程中也显著性增加,消化后粗提物的抑制率可达到49.88%。从而可有效抑制黑色素生成,具有较好的美白潜力。因此,藜蒿叶提取物经体外消化后具有较好的抗氧化活性和抑制酪氨酸酶活力的能力,有望应用于美白产品的开发,该研究为藜蒿茎加工副产物-藜蒿叶的开发提供了借鉴。

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    The changes of phenolic acids content, antioxidant activity and tyrosinase inhibitory activity of Artemisia selengensis Turcz leaves extract in vitro digestion were studied. The results showed that content of chlorogenic acid, 3,4-dicaffeoylquinic acid, 3,5-dicaffeoylquinic acid, 4,5-dicaffeoylquinic acid and 1,5-dicaffeoylquinic acid decreased significantly after simulated digestion. Total phenolic acid content and antioxidant activities including DPPH, FRAP and ABTS increased significantly after in vitro simulated digestion. The contents of total phenolic acid in crude extract and purified sample were increased by 73.57%, 23.79%, respectively. The antioxidant capacity of DPPH, FRAP and free radical scavenging capacity of ABTS in crude extract and purified sample increased by 36.17% and 53.29%, 28.71% and 15.45%, 163.67% and 49.50%, respectively. In addition, the inhibitory rate of Artemisia selengensis Turcz leaves extract on tyrosinase activity increased significantly after in vitro digestion, the inhibition rate of crude extract could reach 49.88%, respectively, which could effectively inhibit melanin production and benefit for whitening. Therefore, Artemisia selengensis Turcz leaves extract exhibits good antioxidant activity and tyrosinase inhibitory activity after in vitro digestion, which has the potential to develop the whitening products. Results in this work provided a reference for the development of Artemisia selengensis Turcz leaves, a by-product of Artemisia selengensis Turcz stem processing.

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熊文,吴婷,曹伟伟,潘思轶,徐晓云.体外消化对藜蒿叶多酚含量及抗氧化活性的影响[J].现代食品科技,2020,36(1):78-83.

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  • 收稿日期:2019-08-27
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  • 在线发布日期: 2020-02-11
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