短链脂肪酸改善2型糖尿病小鼠胰岛素抵抗和胰腺损伤

Short-Chain Fatty Acids Reduced Insulin Resistance and Pancreatic Damage in Type 2 Diabetic Mice

摘要:

以链脲佐菌素(Streptozotocin，STZ)诱导的糖尿病小鼠为研究对象，研究短链脂肪酸对糖尿病小鼠血糖代谢的改善作用。通过腹腔注射200 mg/kg STZ建立糖尿病小鼠模型，研究乙酸钠、丙酸钠和丁酸钠对糖尿病小鼠进食量和体重变化、口服葡萄糖耐量（OGTT）、血糖水平、血清胰岛素水平、胰岛素抵抗（HOMA-IR）、胰岛β细胞功能（HOMA-β）指数及胰腺组织结构的影响。结果显示：与模型组相比，乙酸钠和丙酸钠可显著降低累计进食量（p<0.05），分别降低了10.09%和8.90%；乙酸钠和丁酸钠，对2型糖尿病小鼠其他指标包括体重、空腹血糖、葡萄糖耐量、胰岛素抵抗和胰腺组织损伤修复都没有显著改善作用（p>0.05）。丙酸钠可以显著降低血糖水平（20.65%）和胰岛素抵抗（11.19%），增强胰岛β细胞功能（64.50%），提高葡萄糖耐量，对胰腺组织损伤起到改善作用。

Abstract:

The hypoglycemic effects of short-chain fatty acids (SCFAs) in streptozotocin (STZ) induced diabetic mice were investigated. A diabetic mouse model was established by intraperitoneal injection of 200 mg/kg STZ to examine the effects of sodium acetate, sodium propionate, and sodium butyrate on food intake, body weight, oral glucose tolerance test (OGTT), blood glucose level, serum insulin level, homeostasis model assessment insulin resistance (HOMA-IR), homeostasis model assessment-β (HOMA-β), and pancreatic tissue structure. Compared with untreated model mice, mice receiving sodium acetate or sodium propionate displayed significantly reduced total food intake (p<0.05) (10.09% and 8.90%, respectively). Sodium acetate and sodium butyrate produced no significant improvement in other indices in type 2 diabetic mice (p>0.05), including body weight, fasting glucose, glucose tolerance, insulin resistance, and repair of pancreatic tissue damage. Sodium propionate significantly reduced blood glucose level (by 20.65%) and insulin resistance (by 11.19%) in diabetic mice, and enhanced the function of pancreatic β cells (64.50%) and glucose tolerance, thereby reducing the severity of pancreatic tissue damage.