南极磷虾油灵芝孢子油纳米乳复合物提高小鼠的免疫功能

Nanoemulsion Complex of Euphausia superba (Antarctic Krill) Oil and Ganoderma lucidum Spore Oil Enhances the Immunity of Mice

摘要:

本文研究了乳化溶剂蒸发法制备的南极磷虾油与灵芝孢子油纳米乳复合物对小鼠免疫功能的作用效果。将350只健康雌性小鼠随机分为5批（N=70），每批随机分为7组（n=10），阴性对照组、纳米乳复合物与非纳米乳复合物低（0.25 g/kg·bw）、中（0.50 g/kg·bw）、高（1.50 g/kg·bw）剂量组，分别相当于人体推荐摄入量的5、10、30倍。经口灌胃30 d，每日1次。检测受试物对小鼠各项免疫指标的影响。结果表明，与阴性对照组相比，纳米乳复合物低、中剂量组提高小鼠脾淋巴细胞转化能力分别为35.72%（p<0.05）、157.13%（p<0.01），高剂量组增强小鼠迟发性变态反应效果为32.41%（p<0.05）；中剂量组促进小鼠抗体生成细胞增殖效果为21.12%（p<0.05），低、中、高剂量增强小鼠产生血清溶血素的效果依次为：63.22%（p<0.01）、77.64%（p<0.01）、55.31%（p<0.01）；中、高剂量组增强小鼠NK细胞的活性效果分别为58.92%（p<0.01）和134.83%（p<0.01），且经纳米乳化后效果优于单纯的复合物。南极磷虾油与灵芝孢子油纳米乳复合物对小鼠具有提高免疫力的作用。

Abstract:

The effect of the nanoemulsion complex of Euphausia superba oil and Ganoderma lucidum spore oil on immune function in mice was investigated. Compared with the negative control group, the nanoemulsion complex in the low and medium dose groups improved the splenic lymphocyte transformation capacity by 35.72% (p<0.05) and 157.13% (p<0.01), respectively; in the high dose group, the effect of DNFB-induced delayed allergic reaction in mice was enhanced by 32.41% (p<0.05). The proliferation of mouse antibody-generating cells in the medium dose group was promoted by 21.12% (p<0.05), and the productions of serum hemolysin in mice at low, medium and high doses were enhanced by 63.22% (p<0.01), 77.64% (p<0.01), and 55.31% (p<0.01), respectively. The NK cell activities of mice in the medium and high dose groups were 58.29% (p<0.01) and 134.83% (p<0.01), respectively. Cellular immune function, humoral immune function and NK cell activity were all positive. The nanoemulsion complex of Euphausia superba oil and Ganoderma lucidum spore oil enhanced the immunity of mice.