酵母菌促进单核细胞增生李斯特氏菌的生长

Yeast Promotes the Growth of Listeria monocytogenes

摘要:

为了缩短单核细胞增生李斯特氏菌（单增李斯特氏菌）的前增菌时间，提升检测效率，本研究将酵母菌（ATCC 9763）作为一种生长促进剂加入到单核细胞增生李斯特氏菌的前增菌培养基（LB1）中，结果表明添加适量的酵母菌可以使单增生李斯特氏菌的生长速度提升近100%，并且这种促进作用与培养过程中酵母菌的接种量、培养基的溶氧量以及培养方式密切相关。根据酵母菌的生长特性分析，这种促进作用产生的原因很有可能是由于酵母菌在生长过程中发酵分解了培养基中的糖类等营养物质，改善了单增李斯特氏菌的营养条件，从而提高了单增李斯特氏菌的繁殖速度。本研究为缩短单增李斯特氏菌检测的富集培养时间，提高检测效率奠定了很好的基础，同时也侧面提示我们要关注发酵类食品遭受单增李斯特氏菌污染的食品安全风险。

Abstract:

In order to shorten the propagating time of Listeria monocytogenes and improve the detection efficiency, yeast (ATCC 9763) was added to LB1 medium of Listeria monocytogenes as a growth promoter. The results showed that the growth rate of Listeria monocytogenes could be increased about 100% by adding appropriate amount of yeast. Moreover, the promotion effect was related to the inoculation amount of yeast, dissolved oxygen in the medium and the culture mode. According to the analysis of the growth characteristics of yeast, maybe the degradation of saccharides and other nutrients by yeasts improved the growth environment, increasing the growth rate of Listeria monocytogenes. This result laid a good foundation for shortening the pre-enrichment time and improving the detection efficiency of Listeria monocytogenes. On the other hand, it also suggested that more attention to the food safety risk of fermented food contaminated by Listeria monocytogenes should be paid.