超高效液相色谱-四极杆/静电场轨道阱高分辨质谱快速筛查测定几种食品中的8种碱性色素

Screening of 8 Basic Dyes in Food by Ultra Performance Liquid Chromatography-quadrupole / Electrostatic Field Obitrap High-resolution Mass Spectrometry

摘要:

本文研究了超高效液相色谱-四极杆/静电场轨道阱高分辨质谱法快速测定面包、橙汁、卤豆干等食品中8种碱性色素的方法。样品用乙酸乙酯提取后，以C18（3.0 mm×100 mm，1.8 μm）色谱柱分离，乙腈和10 mmol/L乙酸铵水溶液为流动相梯度洗脱，在正负离子切换模式下检测8种碱性色素。在全扫描模式下提取8种碱性色素的保留时间和一级母离子精确质量数以及同位素丰度比，实现对食品中8种碱性色素的快速测定；以自动触发采集的二级碎片离子精确质量数进行确证。8种碱性线性关系较好，相关系数（R2）大于0.99，回收率在82.2%~105.3%之间，相对标准偏差在6.0%~9.9%之间。该方法简单、快速、可靠，可定性筛查和定量分析食品中8种碱性色素。

Abstract:

A fast and easy method for the quantification of 8 basic dyes in food (bread, orange juice and stewed bean curd) was investigated by ultra performance liquid chromatography-quadrupole/electrostatic field obitrap high-resolution mass spectrometry. The sample was extracted by ethyl acetate, C18 (3.0 mm×100 mm, 1.8 μm) was used as chromatography column to separate the basic dyes with a gradient elution of acetonitrile and 10mmol/L ammonium acetate aqueous solution as the mobile phase. The 8 basic dyes were analyzed in positive and negative mode. The retention time, accurate mass of parent ion and relative isotope abundance were used for fast screening in full scan mode, while accurate mass of fragment ions was used for confirmation in two-stage threshold-triggered full mass scan mode. Under the optimal conditions, good linearities (R2>0.99) were obtained in their respective linear ranges. The recoveries were in the range of 82.2%~105.3% and the relative standard deviation was 6.0%~9.9%. The method was simple, sensitive, accurate and suitable for the rapid screening and confirmation of 8 basic dyes in food.