几种竹叶醇提物体外抗氧化活性的对比

In-vitro Evaluation of Antioxidant Activity of Ethanol Extracts from Several Kinds of Bamboo Leaves

摘要:

采用乙醇提取慈竹、苦竹、四季竹中的有效成分并检测其总黄酮和总蛋白含量，通过测定提取物的DPPH自由基清除能力、还原能力、抑制β-胡萝卜素漂白能力和抑制脂质过氧化能力，比较不同竹叶的抗氧化活性。结果表明：醇提物总蛋白含量大小依次为：苦竹>慈竹>四季竹，总黄酮含量大小依次为：慈竹>苦竹>四季竹。苦竹的DPPH清除能力（IC50=1.53 mg/mL）最强，慈竹的还原能力（IC50=0.55 mg/mL）和抑制脂质过氧化能力（IC50=0.04 mg/mL）最强。三种竹叶DPPH自由基清除率IC50值范围为1.53~2.71 mg/mL，还原能力IC50值范围为0.55~0.97 mg/mL，β-胡萝卜素漂白抑制作用IC50值范围为0.78~0.98 mg/mL，脂质过氧化抑制作用IC50值范围为0.04~0.12 mg/mL。三种竹叶乙醇提取物均具有较好的抗氧化活性，可作为抗氧化剂、防腐保鲜剂或功能性食品添加剂应用于食品工业中。

Abstract:

Ethanol was used to extract the active components from the leaves of cizhu, bitter bamboo and four seasons bamboo, and the contents of total flavonoids and total protein were detected at the same time. DPPH free radical scavenging capacity, reducing capacity, inhibiting beta-carotene bleaching capacity and inhibiting lipid peroxidation capacity of the extracts were measured to compare the antioxidant activities of different bamboo leaves. The results showed that the total protein content in ethanol extracts was ordered as follows: bitter bamboo>cizhu>four seasons bamboo, and the total flavonoids content was ordered as follows: cizhu>bitter bamboo>four seasons bamboo. DPPH scavenging ability of bitter bamboo (IC50=1.53 mg/mL) was the strongest, and the reduction ability (IC50=0.55 mg/mL) and inhibiting lipid peroxidation ability (IC50=0.04 mg/mL) of Cizhu were the strongest. DPPH free radical scavenging rate of the three bamboo leaves ranged from 1.53 to 2.71 mg/mL, reducing capacity from 0.55 to 0.97 mg/mL, beta carotene bleaching inhibition from 0.78 to 0.98 mg/mL, and lipid peroxidation inhibition from 0.04 to 0.12 mg/mL. The ethanol extracts of three kinds of bamboo leaves have good antioxidant activities, which can be used in food industry as antioxidants, preservatives or functional food additive.