盐制女贞子化学成分的UPLC-Q/TOF MS鉴定研究

Study on Chemical Constituents of Ligustri Lucidi Fructus by UPLC-Q/TOF MS

摘要:

采用超高效液相色谱-四极杆飞行时间质谱（UPLC-Q/TOF MS）技术对盐制前后不同形状女贞子的化学成分进行分析，并探讨差异性成分之间的变化规律。采用高效液相色谱法条件：Sharpsil-H C18色谱柱（250 mm×4.6 mm，5 μm），流动相为乙腈-0.1%甲酸水溶液梯度洗脱，柱温25 ℃；流速1 mL/min；进样量5 μL。质谱条件：AB Sciex Triple TOF 5600高分辨串联质谱仪，ESI离子源，正离子与负离子模式下扫描。通过正、负离子质谱信息及元素组成分析，在女贞子肾形生品、肾形盐制品、圆形生品、圆形盐制品的潜在离子中，分别推定出19、21、28、24个化合物。通过UPLC-Q/TOF MS技术，为不同形状的女贞子盐制前后的化学成分分析建立了灵敏度高、分离度高和分析速度快的分析方法，也证明了不同形状的女贞子存在差异性成分，经盐制后成分也存在变化，为后续质量控制和药理研究提供研究基础。

Abstract:

Using a LC-TOF/MS method, the chemical constituents of Ligustri Lucidi fructus were analyzed, and the transformation rule of the different constituents in the processing of Ligustri Lucidi fructus with the salt was discussed. A Sharpsil-H C18 column (250 mm×4.6 mm, 5 μm) was used. The mobile phase was composed of acetonitrile and water with 0.1% formic acid in gradient mode. The flow rate was 1 mL/min, the column temperature was 25 ℃ and the injection volume was 5 μL. An AB Sciex Triple TOF 5600 and ESI ion source was used for mass spectra. The MS data were collected in both positive and negative modes. The result showed that a total of 19 compounds were identified in the kidney-shape Ligustri Lucidi fructus, a total of 21 compounds were identified in the kidney-shape Ligustri Lucidi fructus after processing, a total of 28 compounds were identified in the round Ligustri Lucidi fructus, a total of 24 compounds were identified in the round Ligustri Lucidi fructus after processing. Through UPLC-Q/TOF MS technology, the analytical method of high sensitivity, high degree of separation and quick analysis was established for the chemical composition analysis of the raw and salt products of Ligustri Lucidi fructus. It was also proved that the components in various shapes of Ligustri Lucidi fructus were different in the processing with the salt, which will lay the foundation for the follow-up quality control and pharmacological research.