栀子醇提物对MPTP致帕金森病小鼠多巴胺能神经元的保护作用

Protective Effect of Gardenia jasminoides Extract on Dopaminergic Neurons in Mice with Parkinson's Disease Induced by MPTP

摘要:

本研究以1-甲基-4-苯基-1,2,3,6-四氢吡啶（MPTP）所致帕金森病（Parkinson’s disease，PD）小鼠为模型，系统研究药食两用资源栀子醇提物对PD小鼠多巴胺能神经元保护作用。栀子醇提物（25、50、100 mg/kg）给药小鼠，检测PD小鼠行为学，免疫组化检测黑质多巴胺，研究栀子醇提物对PD小鼠多巴胺能神经元保护作用。与模型组相比，低、中、高剂量组爬杆时间分别减少了24.03%（p>0.05）、26.43%和26.99%（均为p<0.05）；转轴时间分别增加了64.16%、77.46%、86.76%（均为p<0.05）；拉力大小分别增加了16.95%（p>0.05）、22.56%和31.84%（均为p<0.05）。栀子醇提物组TH阳性细胞数目显著高于模型组。与模型组相比，低、中、高剂量组多巴胺（DA）分别增加了100%、138.46%、187.18%（均为p<0.05）；二羟苯乙酸（DOPAC）分别增加了52.63%、89.47%、110.53%（均为p<0.05）。此外，与模型组相比，低剂量组小鼠血清中TNF-α、IL-1β、IL-6含量分别减少了18.30%、23.86%、26.03%（均为p<0.05）。数据显示，栀子醇提物能够显著改善MPTP诱导的PD小鼠行为变化，保护多巴胺能神经元，在治疗PD药物研发方面具有良好的潜力。

Abstract:

In this study, mice with Parkinson's disease (PD) caused by MPTP were used as a model to systematically study the protective effects of Gardenia jasminoides extract from the medicinal and edible resources on the dopaminergic neurons in PD mice. The PD mice were administrated with Gardenia jasminoides extract (25, 50, 100 mg/kg) to detect the behavior. Detection of substantia nigra dopamine in mice by immunohistochemistry, to study the protective effect of Gardenia jasminoides extracts on dopaminergic neurons in PD mice. Compared with the model group, the climbing time of the low, medium and high dose groups decreased by 24.03% (p>0.05), 26.43% and 26.99% (both p<0.05) respectively; the rotational time increased by 64.16% (p<0.05), 77.46% (p<0.05) and 86.76% (p<0.05), respectively. The tensile strength increased by 16.95% (p>0.05), 22.56% (p<0.05) and 31.84% (p<0.05), respectively. The behavior of PD mice was significantly improved after Gardenia jasminoides extract treatments. The number of TH-positive cells in the Gardenia jasminoides extracts group was significantly higher than that in the model group. Compared with the model group, the DA of the low, medium and high dose groups increased by 100% (p<0.05), 138.46% (p<0.05) and 187.18% (p<0.05), respectively. The DOPAC increased by 52.63% (p<0.05), 89.47% (p<0.05) and 110.53% (p<0.05), respectively; In addition, compared with the model group, the serum levels of TNF-α, IL-1β, IL-6 in the low-dose group were reduced by 18.30% (p<0.05), 23.86% (p<0.05) and 26.03% (p<0.05), respectively. The data show that the Gardenia jasminoides extracts can significantly improve MPTP-induced behavioral changes in PD mice, protect dopaminergic neurons, and have good potential in the development of PD drugs.