Abstract:This wok was designed to assess the regulatory potential of Yunnan, a bitter tea extract 1, 3, 4, 9-tetramethyl uric acid (TC) for oxidative stress in diabetic nephropathy mice. The mices were feed by high-fat and high-sugar diet, intraperitoneal injection of cadmium chloride (CdCl2) for 12 weeks to establish an animal model of diabetic nephropathy, and TC for 28 days, to evaluate the changes of serum biochemical indicators. The kidney malondialdehyde (MDA), lipid Oxidation of plasma peroxidation products (LOOH), protein carboxylation (PCO), non-enzymatic antioxidant activities such as superoxide dismutase (SOD), catalase (CAT), and reduced glutathione (GSH) were analyzed to study the effect of cadmium on the degree of oxidative damage in the kidney and tetraethyl uric acid. The results showed that the levels of TG, ALT and LDL-C in the serum of the injury group were significantly up-regulated, 1.88 times, 2.39 times, and 2.86 times, respectively, compared with the blank group. The oxidative indexes MDA, PCO and NO in the tissue were significantly increased by 1.51, 3.88 and 2.03 times, respectively. The antioxidant indexes CAT, SOD and GSH were significantly down-regulated in the injury group, only 68.75%, 77.59%, and 80.95%, respectively. After given TC protective drug, the serum index level was only 68.09%, 49.09%, and 45.65% of that of the injury group; the oxidation index content was decreased to 82.14%, 74.19%, and 64.94% of that of the injury group, which was only; the antioxidant level decreased to 1.41, 1.20 and 1.12 times of that of the injury group. The balance between oxidation and anti-oxidation in cadmium-induced diabetic nephropathy (DN) mice, which leads to an increase in oxidative stress; TC enhanced the antioxidant capacity of antioxidant systems by increasing the activity of antioxidant enzymes. The kidneys were protected together by anti-oxidative stress and anti-inflammatory effects.
