三种多糖降解复合物体外抑制HeLa细胞的增殖作用

Inhibitory Effect of Complex Polysaccharides Degradation Products on Proliferation of HeLa Cells in vitro

摘要:

本文探讨了毛头鬼伞、龙须菜和坛紫菜三种多糖降解复合物体外抑制HeLa细胞增殖的协同效应及其作用机制。将毛头鬼伞、龙须菜和坛紫菜的多糖降解物复配成复合多糖降解物，采用MTT法检测复合多糖降解物对HeLa细胞增殖影响，DCFH-DA检测细胞内活性氧水平，JC-1检测线粒体膜电位变化情况。正交实验结果显示，当毛头鬼伞、龙须菜和坛紫菜三种多糖降解物以质量比为4:4:5组合时，对HeLa细胞的增殖抑制作用最强，在浓度为100 μg/mL时，抑制率达75.72%，远高于三种单味多糖降解物单独使用时的最高抑制率56.09%；DCFH-DA、JC-1检测结果表明，复合多糖降解物可以显著提高细胞内活性氧水平，降低线粒体膜电位。以上结果均说明毛头鬼伞、龙须菜和坛紫菜三种多糖降解物复合后，对HeLa细胞的增殖抑制活性存在协同增效作用，该复合多糖降解物的作用机制可能与提高细胞内活性氧水平和降低线粒体膜电位有关。

Abstract:

The synergistic inhibitory effect of complex polysaccharides degradation products from Coprinus comatus, Gracilaria Lemaneiformis and Porphyra haitanensis on HeLa cell proliferation in vitro and its mechanism were investigated. In the experiment, the complex polysaccharides degradation products were combined by three kinds of polysaccharides degradation products from Coprinus comatus, Gracilaria Lemaneiformis and Porphyra haitanensis. The influence of complex polysaccharides degradation products on HeLa cells proliferation was assayed by MTT test. The intracellular level of reactive oxygen species was determined by DCFH-DA and the change of mitochondrial membrane potential was measured by JC-1. Based on orthogonal experiment, when the quality ratio of three kinds of polysaccharides degradation products from Coprinus comatus, Gracilaria Lemaneiformis and Porphyra haitanensis was 4:4:5, the inhibition effect on proliferation of HeLa cells was the strongest. In a concentration of 100 μg/mL, the inhibition rate was 75.72%, which outclassed the maximum inhibition rate of 56.09% when the three kinds of single polysaccharides degradation product were used alone. The results indicated that treatments of complex polysaccharides degradation products could significantly improve the intracellular level of reactive oxygen species and decrease mitochondrial membrane potential by DCFH-DA and JC-1, respectively. Combination of polysaccharides degradation products from Coprinus comatus, Gracilaria Lemaneiformis and Porphyra haitanensis, exhibited syneigistic the inhibition on proliferation of HeLa cells. The effect of complex polysaccharides degradation products might be related to increasing reactive oxygen species and reducing mitochondrial membrane potential.