可乐碳量子点的提取及其抑菌性研究
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陈巧玲(1986-),女,实验师,硕士研究生,研究方向:食品质量与安全 通讯作者:陈碧桑(1987-),女,实验师,硕士研究生,研究方向:分析化学

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福建省教育厅A类项目(JAT160289)


Extraction and Antibacterial Activity of Coca-Cola Carbon Quantum Dots
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    摘要:

    本研究首次采用浓缩、透析的方法直接从可口可乐中获得碳量子点,并通过透射电镜、紫外分光光度计、红外光谱及荧光分光光度计等方法对其结构进行表征,通过微生物方法对其抑菌性进行研究。结果表明,可乐碳量子点粒度分布窄,单分散性好、表面富含羟基和羧基,溶解度高,对大肠杆菌、金黄色葡萄球菌、白色念珠菌、枯草芽孢杆菌、铜绿假单胞菌等单细胞生物并无抑制效果。另外发现,当可乐碳量子点浓度大于等于100 mg/L时,大肠杆菌和金黄色葡萄球菌的培养基孔洞附近菌落明显增多,可见碳量子点有利于这两种菌的生长,而对其余三种菌并没有明显的影响。本研究所得结果对进一步拓宽碳量子点的合成途径以及可乐碳量子点的后期应用具有重要意义。

    Abstract:

    The carbon quantum dots (CDs) were obtained from Coca-Cola directlyby concentration and dialysisfor the first time in this study. The structure of CDs was characterized by transmission electron microscopy (TEM), ultraviolet spectrophotometer (UV), infrared spectroscopy (IR) and fluorescence spectrophotometer, and the antibacterial activity was investigated by microorganism meathod. The results showed that CDs had a narrow particle size distribution and a good monodispersity, and the surface was rich in hydroxyl and carboxyl groups, which made it have a high solubility. The CDs have no inhibitory effect on single cell organisms such as Escherichia coli, Staphylococcus aureus, Candida albicans, Bacillus subtilis and Pseudomonas aeruginosa. In addition, the colonies of Escherichia coli and Staphylococcus aureus were significantly increased near the pore of culture medium when the concentration of CDs was greater than or equal to 100 mg/L, suggesting that CDs were beneficial to their growth but had no significant effect on the other three kinds of bacteria The results obtained in this study were of great significance for further expanding the synthesis pathway of CDs and the later applicantions.

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陈巧玲,陈碧桑,李飞明,游雨婷,吴秀婷.可乐碳量子点的提取及其抑菌性研究[J].现代食品科技,2018,34(1):52-56.

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  • 收稿日期:2017-08-02
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  • 在线发布日期: 2018-02-02
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