复方苍术方调节肠道菌群及肠道保护作用的研究

Effect of Atractylodes Decoction Prescription on Regulating Gut Flora and Protecting Intestine

摘要:

本文探讨了复方苍术方调节肠道菌群及肠保护作用。给予复方苍术方后，中、高剂量组肠球菌及高剂量组肠杆菌菌落数（按lg CFU计）分别降低6.35%、8.17%及5.69%；中、高剂量组乳杆菌和双歧杆菌菌落数分别增加7.04%、6.74%、6.99%和8.10%，且高于阳性对照组；同时，中、高剂量组盲肠内容物pH值分别降低6.58%和5.26%；肠道组织病理结果显示复方苍术方低剂量组十二指肠、回肠及中、高剂量组十二指肠、空肠、回肠A/V比值分别增加29.84%、17.47%、56.01%、31.18%、20.09%、46.51%、30.52%和20.09%，且中、高剂量组空肠、回肠A/V比值明显高于阳性对照组。此外，复方苍术方低、中、高剂量组小肠墨汁推进率分别增加43.92%、69.93%和98.31%。研究结果表明，复方苍术方可能通过调节肠道菌群结构改善肠道微环境，且效果优于单用低聚半乳糖。

Abstract:

Effects of Atractylodes Decoction prescription on regulating gut flora and protecting intestine were investigated in this study. After treatment with Atractylodes Decoction prescription for 30 days, compared with pre-treated animals, the intestinal Escherichia coli in high-dose group and enterococcus in middle- and high-dose groups were significantly decreased by 8.17%, 6.35% and 5.69%, respectively. Conversely intestinal bifidobacteria and lactobacillus in middle- and high-dose groups were increased significantly by 7.04%, 6.74%, 6.99% and 8.10%, respectively. Furthermore, when compared with galactooligosaccharide (GOS) group, the effect of Atractylodes Decoction prescription on intestinal bifidobacteria and lactobacillus were also significantly increased. But the pH value of cecal contents in middle-dose and high-dose groups were noticeably reduced by 6.58% and 5.26%, respectively. In the histopathological examination of intestinal tract, the ratio of the villus length to the corresponding crypt depth (A/V ratio) of duodenum and ileum in low-dose group, A/V ratio of duodenum, jejunum and ileum in middle- and high-dose groups, were markedly improved by 29.84%, 17.47%, 56.01%, 31.18%, 20.09%, 46.51%, 30.52% and 20.09%, respectively. In addition, the A/V ratio of the jejunum and ileum in middle- and high-dose groups were markedly increased when compared with the GOS group. In addition, the intestinal propulsion rates in treated animals were dose-dependently promoted by 43.92%, 69.93% and 98.31%, respectively. These data demonstrated that Atractylodes Decoction prescription improve intestinal microenvironment by regulating intestinal flora and showed improving effects when compared with GOS alone.