双酚S暴露对秀丽隐杆线虫行为学及生长发育的影响

Effects of Bisphenol S Exposure on Behavior and Growth of Caenorhabditis elegans

摘要:

双酚S（BPS）作为双酚A（BPA）的替代物，在各个产品中(奶瓶、食品罐和热敏纸等)已经得到广泛应用，但是目前关于BPS的毒理学研究甚少。本文以BPS为研究对象，秀丽线虫为模式生物，分析其对运动行为能力及生长发育的影响。研究结果表明，急性毒性试验中，暴露浓度达到1 μM时，运动行为学指标身体弯曲频率显著下降，暴露浓度达到10 μM时，运动行为学指标头部摆动频率显著下降；随着浓度的增加，BPS还会影响秀丽隐杆线虫的寿命和子代数目，导致寿命缩短和子代数目的减少，BPS浓度达到100 μM时，与对照组相比，寿命下降了35.42%，子代数目下降了30.47%；BPS暴露会导致秀丽线虫体内活性氧自由基含量显著上升，推测高浓度的BPS可能造成秀丽线虫的氧化损伤，进而影响运动行为能力、寿命及子代数量。

Abstract:

Bisphenol S (BPS) has been widely used as a substitute for bisphenol A (BPA) in various products (bottles, food cans, thermal paper, etc.), but there were few studies on toxicology of BPS. In this study, BPS was used as the research object, and Caenorhabditis elegans (C. elegans) was used as model organism to analyze its effect on the motor behavior and growth of C. elegans. The results showed that when the exposure concentration reached 1μM, the frequency of body movement was significantly decreased. And when the exposure concentration reached 10 μM, the thrashing frequency of the head was significantly decreased in the acute toxicity test. With the increase of concentration, BPS also affected the life span and the number of progeny of C. elegans, resulting in shortened life span and fewer brood size. When the concentration of BPS reached 100 μM, the life span decreased by 35.42% and the brood size decreased by 30.47% as compared with the control group; BPS exposure could lead to a significant increase of the reactive oxygen species (ROS) free radicals content in the C. elegans, suggesting that high concentrations of BPS might cause oxidative damage to the C. elegans, and further affect the motor behavioral capacity, life span and the number of offspring.