红松壳多酚、黄酮和多糖含量及抗氧化活性相关性的研究

Study on the Correlation between Contents of Polyphenols, Flavonoids, Polysaccharides in Pinus koraiensis Seed Putamina and Their Antioxidant Activities

摘要:

为了探究红松壳中主要活性成分的含量及其与抗氧化活性之间的相关性，试验以伊春红松壳为原料，使用不同浓度乙醇溶剂提取红松壳多酚、黄酮和多糖并分析其含量，采用SPSS软件分析松壳中活性物质与抗氧化活性之间的相关性。试验结果表明，当乙醇的浓度为40%时，红松壳多酚、黄酮和多糖得率均最高，分别为4.05 mg/g、1.7 mg/g和7.05 mg/g。抗氧化活性试验表明，红松壳多酚抗氧化活性显著高于黄酮和多糖，与Vc抗氧化活性相当。其中松壳多酚对DPPH·的IC50（半数清除率浓度）值是0.20±0.05 μg/mL，对·OH的IC50值是13.04±0.04 μg/mL，对总还原力的IC50值是40.15±0.06 μg/mL。相关性分析结果也表明松壳多酚与抗氧化活性相关性最高，其中松壳多酚与DPPH·相关性是0.908，与·OH相关性是0.989，与总还原力相关性是0.989（p<0.01），可以看出松壳多酚是主要的抗氧化成分。本实验研究的结果为伊春红松壳副产物的综合利用以及开发天然抗氧化剂提供了科学依据。

Abstract:

In order to investigate the correlation between contents of major active ingredients in Pinus koraiensis seed putamina and its antioxidant activity, Yichun Pinus koraiensis seed putamina was used as raw material in this study to extract polyphenols, flavonoids and polysaccharides by different ethanol concentrations. The correlation between active ingredients in Pinus koraiensis seed putamina and their antioxidant activities were analyzed by SPSS statistical analysis software. The results showed that when the concentration of ethanol was 40%, the highest yields of polyphenols, flavonoids and polysaccharides in Pinus koraiensis seed putamina were 4.05 mg/g, 1.7 mg/g and 7.05 mg/g, respectively. Antioxidant activity test showed that antioxidant activity of polyphenols from Pinus koraiensis seed putamina was significantly higher than that of flavonoids and polysaccharides, which was comparable to that of Vc. The IC50 (half clearance concentration) values of polyphenols from Pinus koraiensis seed putamina on the 1,1-diphenyl-2-picrylhydrazyl (DPPH), hydroxyl radical (·OH) and the total reducing power were 0.20±0.05 μg/mL, 13.04±0.04 μg/mL and 40.15±0.06 μg/mL, respectively. The correlation analysis showed that the correlation between the polyphenols from Pinus koraiensis seed putamina and the antioxidant activity was the highest, and the correlations between the polyphenols from Pinus koraiensis seed putamina and DPPH,·OH as well as the total reducing power were 0.908, 0.989 and 0.989 (p<0.01), respectively. It could be seen that polyphenols from Pinus koraiensis seed putamina were the main antioxidant components. The results of this study provided a scientific basis for the comprehensive utilization of the by-products of the Yichun Pinus koraiensis seed putamina and the development of natural antioxidants.