魔芋葡甘露聚糖和香蕉抗性淀粉对小鼠肠道短链脂肪酸分泌及血脂影响的研究

Effects of Konjac Glucomannan and Banana-resistant Starch on Intestinal Secretion of Short-chain Fatty Acids and Blood Lipids in Mice

摘要:

本研究意在探索一种KGM与BRS复合的益生元，最终获得一种较好的有维持及增强肠道和心血管健康的益生元制剂。以健康雄性BALB/c小鼠为模型，设定对照组和6个试验组，设计制造三组不同添加剂量的BRS和三组不同配比BRS+KGM的AIN93 纯化饲料饲喂小鼠，4周后测定小鼠盲肠SCFA含量和血脂指标。在SCFA分泌水平研究中，BRS组1、组2和BRS+KGM组2小鼠盲肠内容物中丙酸的分泌量显著增加（p <0.05），而BRS组3和BRS+KGM组2乙酸、丁酸和总SCFA的分泌增加量极显著（p <0.01），BRS+KGM组3丁酸分泌量明显增加（p<0.01）。在血脂水平研究中，BRS组3HDL-C的含量极显著升高（p<0.01），在BRS+KGM组2和组3中，均为TG、TC含量分别极显著（p<0.01）、显著（p<0.05）降低，HDL-C含量显著升高（p<0.05）。BRS和KGM对SCFA的分泌水平和血脂水平均表现明显的剂量依赖性。综合比较，BRS+KGM组2复合制剂（5%KGM +23.5%BRS）为本研究中最佳复合益生元。

Abstract:

A type of prebiotic combining konjac glucomannan (KGM) and banana-resistant starch (BRS) was evaluated, to yield an optimized prebiotic formulation that could maintain and improve intestinal and cardiovascular health. Healthy male BALB/c mice were used as models, and one control group and six experimental groups were established. AIN93 purified diets classified into three groups with different amounts of added BRS and three groups with different proportions of BRS and KGM were designed and fed to the mice. Four weeks later, the cecal short-chain fatty acid (SCFA) levels and blood lipid indices in mice were determined. In the analysis of SCFA levels, the secretion of propionic acid in cecal contents of the mice in group BRS 1, group BRS 2, and group BRS + KGM 2 increased significantly (p < 0.05). Additionally, the secretions of acetic acid, butyric acid, and total SCFAs in group BRS 3 and group BRS + KGM 2 were also significantly increased (p < 0.01), as was butyric acid secretion in group BRS + KGM 3. In the analysis of blood lipid levels, the high-density lipoprotein-cholesterol (HDL-C) levels were significantly increased in group BRS 3 (p < 0.01). In both groups BRS + KGM 2 and BRS + KGM 3, levels of total cholesterol (TC) and triglycerides (TGs) were significantly decreased (p < 0.05 and p < 0.01, respectively), whereas HDL-C levels were significantly increased (p < 0.05). In conclusion, SCFA secretion levels and blood lipid levels were altered in a dose-dependent manner in response to BRS and KGM consumption. The comprehensive comparisons performed in this study revealed that the compound formulation (5% KGM + 23.5% BRS) of group BRS + KGM 2 was the best composite prebiotic formulation.