翡翠贻贝多糖的分离及其对高脂饮食小鼠抗氧化活性的影响

Isolation of Polysaccharides from Perna viridis and Their Antioxidant Activities in High-fat Diet-fed Mice

摘要:

已有的研究表明：翡翠贻贝粗多糖（PVP）对高脂模型小鼠具有显著的降低血脂作用，本文对翡翠贻贝粗多糖进行了纯化与分析，并研究了其对高脂模型小鼠体内抗氧化作用的影响。PVP经DEAE-52与Sephadex柱层析分离得到两个主要级分P1和P2。经化学分析，P1和P2均为有硫酸基的酸性多糖，相对分子质量分别为6.89×105 Da和2.87×105 Da。氨基葡萄糖：糖醛酸：氨基半乳糖的百分比例分别为：3.7:2.6:1、2.8:1.6:1。以翡翠贻贝粗多糖低[50mg/(kg?d)]、中[100mg/(kg?d)]、高[200mg/(kg?d)]剂量灌喂高脂饮食小鼠（日常饮食中添加2%胆固醇）。结果显示：PVP中、高剂量组能显著增强高脂模型小鼠血清、肝脏和大脑中的T-AOC、GSH-Px, SOD 和CAT活性，且降低MDA含量(p﹤0.05或p <0.01)。研究表明PVP可能通过激活高脂小鼠体内抗氧化酶活性、降低体内过氧化水平而起到降低血脂作用。

Abstract:

Previously, the Perna viridis polysaccharides (PVPs) were demonstrated to have a remarkable hypolipidemic effect in a mouse model of hyperlipidemia. Herein, the PVPs were purified and analyzed, and their antioxidant effects were evaluated in a mouse model of hyperlipidemia. Two main fractions P1 and P2 were separated from PVPs by DEAE-52 and Sephadex G-200 column chromatography. Chemical analysis revealed that P1 and P2 were acidic polysaccharides with sulfate groups, and their relative molecular weights (MWs) were 6.89 × 105 Da and 2.87 × 105 Da, respectively. The content ratios of glucosamine, uronic acid, and galactosamine for P1 and P2 were 3.7:2.6:1 and 2.8:1.6:1, respectively. High-fat diet-fed mice (a diet supplemented with 2% cholesterol) were intragastrically administered low dose [50mg/(kg?d)], medium dose [100mg/(kg?d)], and high dose of PVPs [200mg/(kg?d)]. The results showed that the medium dose and high dose of PVPs could significantly enhance the total antioxidant capacity (T-AOC), as well as glutathione peroxidase (GSH-Px), superoxide dismutase (SOD), and catalase (CAT) activities and reduce the malondialdehyde (MDA) content in serum, the liver, and the brain of the hyperlipidemic mouse model (p < 0.01) or (p < 0.05). The results demonstrate that PVP can exert a hypolipidemic effect by activating the activities of antioxidase in vivo and by reducing the level of peroxidation in hyperlipidemic mice.