EGCG磷脂复合物抗氧化活性的研究

Antioxidant Activity of (-)-Epigallocatechin Gallate-phospholipid Complex

摘要:

本文研究了表没食子儿茶素没食子酸酯磷脂复合物的体外抗氧化活性。将EGCG和大豆卵磷脂溶于无水乙醇，制备得到EGCG磷脂复合物。通过高效液相色谱(HPLC)测定，发现制备得到的EGCG磷脂复合物中EGCG的复合率大于99%，并利用紫外扫描、红外光谱、X射线衍射等方法对其结构进行表征，说明通过本实验方法制备得到了目标复合物。通过DPPH、FRAP、TEAC等方法测定EGCG磷脂复合物的体外抗氧化活性，结果表明EGCG磷脂复合物的体外抗氧化能力显著高于VE（P<0.05）。同时测定添加EGCG磷脂复合物后，不同贮藏时间（3、6、9、12和15 d）反映大豆油氧化的指标。结果表明EGCG磷脂复合物对于大豆油的氧化具有抑制作用且呈现剂量依赖关系，并且在贮藏15 d时，抗氧化效果显著好于BHT（P<0.05）。研究表明，制备得到的EGCG磷脂复合物具有显著的抗氧化活性，并且可以有效抑制大豆油的氧化。

Abstract:

The in vitro antioxidant activity of (-)-epigallocatechin gallate (EGCG)-phospholipid complex (EPC) was studied. First, EPC was prepared by dissolving EGCG and soybean lecithin in ethanol. High-performance liquid chromatography showed that the complexation rate of EGCG in EPC was more than 99%, and the structure of EPC was characterized by ultraviolet spectroscopy, infrared spectroscopy, and X-ray powder diffractometry. The in vitro antioxidant activity of EPC, as evaluated by diphenylpicrylhydrazyl and ferric reducing ability of plasma and Trolox equivalent antioxidant capacity methods, was found to be significantly higher (p < 0.05) than that of VE. Indicators reflecting the oxidation of soybean oil at different storage times (3, 6, 9, 12, and 15 days) were also evaluated after the addition of EPC. The results showed that the inhibitory effect of EPC on the oxidation of soybean oil was dose-dependent as well as significantly higher (p < 0.05) than that of butylated hydroxytoluene after a 15-day storage. These results suggest that the prepared EPC has strong antioxidant activity and can effectively inhibit the oxidation of soybean oil.