桉叶多酚对大鼠乙醇急性氧化损伤保护作用的研究

Protective Effect of Eucalyptus Polyphenols on Acute Ethanol-induced Oxidative Damage in Rats

摘要:

为了评价桉叶多酚对昆明大鼠体内乙醇急性氧化损伤的保护效果，本文以桉叶多酚饲喂昆明大鼠，以乙醇灌喂大鼠进行急性氧化损伤造模，对血液和肝脏总抗氧化能力、各抗氧化蛋白酶活性和肝脏组织病变情况进行检测。结果显示：体内总抗氧化能力提升，其中血清最高增强33.12%，肝脏最高增强56.83%；能有效降低体内MDA的含量，其中血清最高降低23.89%，肝脏最高降低50.73%；T-SOD活性有一定程度增强；实验组GSH-Px活性有所降低，但是给药组与阳性对照组无差异；肝脏切片观察发现，三个给药组肝脏组织的细胞排列规则，不规则空泡减少，肝小叶炎性细胞浸润较少，少见有局部坏死灶的出现，表明乙醇急性氧化损伤受到抑制，肝脏组织得到保护。综合所述，桉叶多酚具有体内抗氧化保护作用，这为桉叶多酚作为抗氧化剂的应用提供了研究基础。

Abstract:

To evaluate the protective effect of Eucalyptus polyphenols on acute ethanol-induced oxidative damage in Kunming rats, Eucalyptus leaves were fed to Kunming rats, and a rat model of acute oxidative damage was established by ethanol feeding. The total antioxidant capacities (T-AOC) of the blood and liver, activity of each antioxidant enzyme, and liver tissue lesions of the rats were examined. Eucalyptus polyphenols enhanced the total antioxidant capacity, to a maximum of 33.12% and 56.83% in the serum and liver, respectively. The malondialdehyde (MDA) content was effectively reduced; the largest reductions in the serum and liver were 23.89% and 50.73%, respectively. The total superoxide dismutase (T-SOD) activity was increased to some extent. The glutathione peroxidase (GSH-Px) activity was decreased in the experimental group, but there was no significant difference from the positive control group. The observation of liver slices showed that the cells of three liver tissues from the experimental group were arranged regularly, the number of irregular vacuoles was reduced, and lobular inflammatory cell infiltrations and local necrosis were rarely found, indicating that acute ethanol-induced oxidative damage was inhibited and the liver tissue was protected by Eucalyptus polyphenols. In summary, Eucalyptus polyphenol confers in vivo antioxidant protection, which provides a research basis for the use of Eucalyptus polyphenols as an antioxidant.