平榛叶中黄酮类化合物成分的研究

A Study of Flavonoids from the Leaves of Corylus heterophylla Fisch.

摘要:

利用高效液相色谱电喷雾离子化串联二级质谱（HPLC-ESI-MS/MS）技术，根据保留时间、母离子和二级质谱碎片与标准品或参考文献比对，对平榛叶黄酮成分进行了定性分析；通过多反应监控模式（MRM）对平榛叶黄酮成分进行了定量分析。共从中鉴定出12种化合物，其中11种为首次从平榛叶中发现，分别为表儿茶素（41.45±1.87 μg/g，干叶）、绿原酸（3-咖啡酰奎宁酸，389.00±17.15 μg/g）、丁香亭-3-O-葡萄糖苷（6169.78±209.73 μg/g）、丹酚酸C（443.37±21.07 μg/g）、金圣草黄素-6-C-葡萄糖苷（206.81±10.07 μg/g）、金丝桃苷（槲皮素-3-O-半乳糖苷，154.44±7.63 μg/g）、槲皮苷（槲皮素-3-O-鼠李糖苷，937.15±40.86 μg/g）、西伯利亚落叶松黄酮-3-O-半乳糖苷（17.27±0.82 μg/g）、锦葵色素-3-O-葡萄糖苷（202.74±10.04 μg/g）、西伯利亚落叶松黄酮-3-O-葡萄糖苷（101.27±4.99 μg/g）和山奈酚-3-O-鼠李糖苷（112.37±4.77 μg/g）。

Abstract:

Qualitative analysis of flavonoids in the leaves of Corylus heterophylla Fisch. was performed using high-performance liquid chromatography coupled with electrospray ionization tandem mass spectrometry (HPLC-ESI-MS/MS). Compounds were identified by comparison of retention times, parent ions, and secondary ion fragments. Quantitative analysis of the flavonoids was performed using HPLC-ESI-MS/MS in the multiple reactions monitoring (MRM) mode. The results showed that 12 compounds were found in the leaves of C. heterophylla, and 11 compounds were reported for the first time, namely epicatechin (41.45 ± 1.87 μg/g, dry weight), chlorogenic acid (3-caffeoylquinic acid, 389.00 ± 17.15 μg/g), syringetin-3-O-glucoside (6169.78 ± 209.73 μg/g), salvianolic acid C (443.37 ± 21.07 μg/g), chrysoeriol-6-C-glucoside (206.81 ± 10.07 μg/g), hyperoside (quercetin-3-O-galactoside, 154.44 ± 7.63 μg/g), quercitrin (quercetin-3- O-rhamnoside, 937.15 ± 40.86 μg/g), laricitrin-3-O-galactoside (17.27 ± 0.82 μg/g), malvidin-3-O-glucoside (202.74 ± 10.04 μg/g), laricitrin-3- O-glucoside (101.27 ± 4.99 μg/g), and kaempferol-3-O-rhamnoside (112.37 ± 4.77 μg/g).