竹叶黄酮和决明子复配与维生素C、E协同调节高脂血症大鼠脂代谢及抗氧化应激的研究

Regulation of Lipid Metabolism and Oxidative Stress by Bamboo Leaf Flavonoid and Cassia Seed Compound Associated with Ascorbyl Palmitate and Vitamin E Succinate in Hyperlipidemia Rats

摘要:

本文研究了[竹叶黄酮（BLF）+决明子粉（CSF）=16:9]与维生素C棕榈酸酯（AP）、维生素Ｅ琥珀酸酯（α-TOS）的体外抗氧化活性，并考察了不同配比组方调节高血脂大鼠脂质代谢及其抗氧化应激的能力。[BLF+CSF]与AP组合（5:1）的体外抗氧化协同增效作用显著强于[BLF+CSF]与α-TOS组合（5:1）；[BLF+CSF]与AP、α-TOS组合（5:1:1）协同增效效果最为显著，表现出最强的体外抗氧化能力。构建高脂血大鼠模型，并评价不同组合物降脂和抗氧化应激能力，结果表明各组方均能够调节高脂血症大鼠的脂质代谢，提高其抗氧化应激能力。其中，[BLF+CSF]与AP和α-TOS（5:1:1）协同增效调节脂质代谢和抗氧化应激效果最为显著，可协同增效显著降低动脉粥样硬化指数（p<0.01），调控血脂：降低TC（p<0.01）、TG（p<0.01）、LDL-c（p<0.01），显著升高HDL-c（p<0.01）。在体内抗氧化应激能力上：显著提高血清T-AOC（p<0.01），显著增加SOD活性（p<0.01）、GSH-Px活性（p<0.01），降低MDA（p<0.01）。

Abstract:

This study was to investigate the in vitro oxidative stress activity of bamboo leaf flavonoid (BLF) and Cassia seed powder (CSF) compound (16:9, m/m), in combination with ascorbyl palmitate (AP) and Vitamin E succinate (α-TOS), as well as their ability to regulate lipid metabolism and anti-oxidative stress at different ratios in hyperlipidemic rats. Combination of [BLF +CSF] and AP with ratio of 5:1) had synergistic anti-oxidative activity, which was significantly higher than that of [BLF+CSF] and α-TOS with ratio of5:1. The combination of [BLF+CSF], AP, and α-TOS with ratio of 5:1:1 exhibited the most significant synergistic effect. A hyperlipidemia rat model was established, and the lipid-lowering and anti-oxidant abilities of different combinations were evaluated. The results showed that all combinations could effectively regulate lipid metabolism and reduce oxidative stress in hyperlipidemic rats. The combination of [BLF+CSF], AP, and α-TOS (5:1:1) had the strongest synergistic effect in regulating lipid metabolism and oxidative stress. The synergistic effect included a significant reduction in atherosclerosis index and regulation of blood lipids. Total cholesterol (p < 0.01), triglycerides (p < 0.01), and low density lipoprotein cholesterol (p < 0.01) were reduced, and high density lipoprotein cholesterol was increased (p < 0.01). In terms of oxidative stress, the combination significantly increased serum total antioxidant capacity (p < 0.01), superoxide dismutase activity (p < 0.01), and glutathione peroxidase activity (p < 0.01), and reduced malondialdehyde content (p < 0.01).