波纹巴非蛤多糖对高脂模型小鼠血脂的影响

Effect of Polysaccharide From Paphia undulate on Blood Lipid of Hyperlipidemia Model Mice

摘要:

本文对波纹巴非蛤多糖进行纯化分析并研究了其对高脂模型小鼠血脂的影响。波纹巴非蛤粗多糖（PUG-1）经DEAE-52纤维素柱层析后，得到PUG-1a、PUG-1b两个主要级分，总糖胺聚糖含量分别为68.3%和62.8%，两个级分组成中氨基葡萄糖:氨基半乳糖:己糖醛酸:硫酸基比例分别为：1.8:0.9:1.3:1.0和1.5:0.8:0.9:1.0；相对分子质量分别为30000 Da和10000 Da。红外光谱分析显示具有糖胺聚糖的特征基团吸收峰。体内实验采用高脂饮食建立小鼠高脂模型, 同时给药组灌服高、中、低（400、200、100 mg/kg?d）3个剂量的波纹巴非蛤多糖溶液（PUG-1）和阳性药物（辛伐他汀，0.2 mg/kg?d），4周后检测各组小鼠血清中TC、TG、LDL-c、HDL-c的含量。PUG-1低、中、高三个剂量组均能显著降低高脂模型小鼠的TC、TG、LDL-c水平与LDL-c/HDL-c（p<0.05或p<0.01），中高剂量组能提高HDL-c水平（p<0.05），提示波纹巴非蛤多糖PUG-1对高脂模型小鼠具有较好的降低血脂与预防动脉粥样硬化作用。

Abstract:

Polysaccharides (PUG-1) were prepared from Paphia undulate by protease hydrolysis, and the effect of PUG-1 on blood lipid of hyperlipidemia model mice was investigated. PUG-1 was separated to two fractions named PUG-1a and PUG-1bby DEAE-52-cellulose exchange chromatography, with relative molecular weight of 30.0 kDa and 10.0 kDa respectively. The glycosaminoglycan contents of PUG-1a and PUG-1b were 68.3% and 62.8%, respectively. And the ratio of gucosamine, galactosamine, hexuronic acid and sulfate in PUG-1a and PUG-1b were 1.8:0.9:1.3:1.0 and 1.5:0.8:0.9:1.0, respectively. FT-IR spectrum showed the characteristic absorption of glycosaminoglycan. The hyperlipidemia model mice were established by feeding with high fat diet (basal laboratory diet with 2% cholesterol). Meanwhile, the mice were administered PUG-1 orally at dosage of 400, 200 and 100 mg/kg?d, respectively. Normal control and positive control group were treated with distilled water and Simvastatin (0.2 mg/kg?d). The contents o f total cholesterol (TC), triglyceride (TG), low density lipoprotein-cholesterol (LDL-c) and high density lipoprotein-cholesterol (HDL-c) in serum were examined after four weeks. The PUG-1 decreased the contents of TC, TG and LDL-c in serum and increased HDL-c significantly (p<0.05 or p<0.01) in the experimental hyperlipidemic mice. These results suggest that PUG-1 can modulate the level of blood lipid in hyperlipidemia mice and can be prevention of atherosclerosis.