灵芝深层发酵中麦角甾醇含量的测定

Determination of Ergosterol by HPLC in the Fermentation of Ganoderma lucidum

摘要:

利用HPLC法研究了灵芝真菌发酵过程中胞内外麦角固醇的含量，经紫外全波长扫描，确定测定波长为282 nm，研究表明：此方法测定麦角固醇含量最大标准偏差为0.92%，得到线性回归方程为y=28685x-382.61（R=0.9998），该回归方程在20~500 μg的范围内。精密度试验，灵芝真菌发酵胞内外麦角固醇含量RSD分别为0.40%和1.96%，说明此试验设计条件精密度良好，经加标后，麦角固醇的回收率在96.82~102.50%之间，表明此方法用于测定灵芝真菌发酵过程中麦角固醇含量准确、可行，经测定灵芝真菌发酵至120 h，麦角固醇含量增至最高，胞内含量达到0.64 mg/g，胞外含量达到0.07 mg/mL。

Abstract:

The ergosterol content in fermentation of Ganoderma lucidum was determined by HPLC at 282 nm. The results showed that the maximum standard deviation was 0.92% and the linear regression equation y= 28685x-382.61 (R=0.9998) in the range of 20~500 μg. The precision test showed that Ganoderma lucidum extracellular and intracellular ergosterol content of RSD were 0.40% and 1.96%, respectively. The spike recoveries for ergosterol were between 96.82 and 102.50%. The method was feasibility and accurate for determination of ergosterol content in fermentation of Ganoderma lucidum. The ergosterol content of Ganoderma lucidum extracellular and intracellular were 0.07 mg/mL and 0.64mg/g, respectively, after fermentation for 120 h.