响应曲面分析法优化苹果渣中多酚类物质的果胶酶辅助提取工艺

Optimization of Pectinase Aided Polyphenol Extraction from Apple Pomace by Response Surface Methodology

摘要:

研究了响应面分析法对苹果渣中多酚类物质的果胶酶辅助提取工艺。以总多酚、咖啡酸提取量为评价指标，在单因素实验基础上根据中心组合实验设计原理，采用3因素5水平的响应曲面分析法对提取工艺进行了优化。通过响应曲面实验及回归分析得出最佳工艺条件为：果胶酶/基质比0.11、反应温度38.00 ℃、反应时间12.53 h。在此条件下，预测总多酚和咖啡酸提取量分别为11.32 g GAE/kg和173.87 mg/kg。经验证，总多酚和咖啡酸提取量分别达到11.08 g GAE/kg和170.03 mg/kg。另外，相对于对照组的6.78 g GAE/kg和13.14 mg/kg分别提高1.6倍和12.9倍。表明由响应曲面法获得的二次模型方程能较好地预测实验结果及优化条件；果胶酶辅助提取工艺稳定合理、准确可靠，是提取苹果渣中总多酚、咖啡酸的可行方法。

Abstract:

In order to enhance the extraction of polyphenol from apple pomace, carbohydrate-hydrolyzing enzyme (pectinase) aided polyphenol and caffeic acid extraction techniques were studied with response surface methodology. The optimum conditions were as follows: the ratio of pectinase to substrate 0.11, the reaction temperature 38.00 ℃, and the reaction time 12.53 h. Under the optimal conditions, the predicted value of total phenolic content and caffeic acid content were 11.32 g GAE/kg and 173.87 mg/kg, respectively. The experimental values of total phenolic content (11.08 g GAE/kg) and caffeic acid content (170.03 mg/kg) of the apple pomace were well matched with the response surface methodology predicted values. In addition, the levels of total phenolic content and caffeic acid content obtained from optimal conditions were about 1.6 and 12.9 folds greater, respectively than those of the control treatment. It was a feasible method to extract the polyphenol from apple pomace.