香蕉粉干预对糖尿病大鼠肠道菌群调节作用研究

Influence of Raw Banana Powder on Intestinal Flora of Diabetic Rats

摘要:

观察香蕉粉干预对糖尿病大鼠肠道菌群的影响。将SD大鼠分为：正常对照组（NCG）、糖尿病对照组（DCG）、低剂量组（LDG）、中剂量组（MDG）和高剂量组（HDG），干预4周后检测5种常见肠道菌群的分布。结果表明：干预后高剂量组（263.50±8.71 g）和中剂量组（258.25±11.89 g）的体重显著低于糖尿病对照组（278.75±5.06 g）；各干预组的排便量（182.57±12.80 g、188.00±17.09 g、163.00±8.75 g）显著高于糖尿病对照组（146.57±20.44）；香蕉粉可显著增加双歧杆菌、拟杆菌、乳酸杆菌的菌落数，显著减少肠球菌菌落数，各干预组的B/E值（1.17±0.15、1.24±0.08、1.21±0.11）显著高于糖尿病对照组（1.13±0.07）；各干预组的短链脂肪酸含量（188.50±46.90 mmol/L、118.60±32.41 mmol/L、61.42±10.80 mmol/L）显著高于糖尿病对照组（32.46±8.34 mmol/L），同时pH值显著降低。香蕉粉有控制糖尿病大鼠体重增加和促进排便的作用，明显改善肠道菌群结构，并增加其酵解产物，降低肠道pH值，从而发挥其对机体的健康作用。

Abstract:

To investigate the influence of raw banana powder (RBP) on intestinal floras, SD rats were divided into 4 groups: normal control group (NCG, n=8), low-dose group (LDG, n=8), middle-dose group (MDG, n=8), and high-dose group (HDG, n=8). While gastric perfusion of distilled water was given to those in NCG and DCG. Collect the feces before and after the 4-week intervention to measure the distribution of intestinal flora (Escherichia coli, Bacillus subtilis, Enterococci, Bifidobacterium, Lactobacillus). Then the colon contents of the rats were collected, and their pH value and short chain fatty acid (SCFA) content were measured. Results showed that RBP can help to control weight gain. The body weight of HDG and MDG were 263.50±8.71 g and 258.25±11.89 g, respectively, after intervention, significantly below DCG (278.75±5.06 g). RBP had the effect of promoting defecation. The defecation amounts of the intervention groups (182.57±12.80 g, 188.00±17.09 g and 163.00±8.75 g) were significantly higher than that in DCG (146.57±20.44 g). RBP can increase the colony number of Bifidobacterium, Lactobacillus and Bacillus subtilis, reduce the number of Enterococci falls and increase the B/E value. And the B/E values of the intervention groups (1.17±0.15, 1.24±0.08 and 1.21±0.11) were significantly higher than DCG (1.13±0.07). RBP can increase SCFA contents of different groups (188.50±46.90 mmol/L, 118.60±32.41 mmol/L and 61.42±10.80 mmol/L) and decrease the pH value of colon content in the intervention groups. Therefore, RBP can significantly improve the intestinal flora, increase their glycolytic products SCFA and reduce intestinal pH value.