中草药提取液对采后西兰花主要致腐菌的抑制作用研究

Inhibitory Effects of Chinese Herbal Extracts on Postharvest Fungul Rot of Broccoli

摘要:

为探讨中草药提取液对采后西兰花致腐菌芸苔生链格孢菌和核盘菌的抑制效果，采用生长速率法、滤纸片法对不同中草药提取液的抑菌效果进行对比，然后利用西兰花活体验证试验做进一步验证，筛选出抑菌效果较好的中草药种类，并进一步通过体积浓度梯度稀释法，对其最小抑菌浓度（MIC）和最小杀菌浓度（MBC）进行了探讨。结果表明：不同天然植物提取液对采后西兰花主要致腐微生物链格孢菌和核盘菌均有不同程度的抑制作用，其中山豆根、肉豆蔻提取液的抑菌效果较强。山豆根、肉豆蔻提取液对链格孢菌的MIC均为占培养液体积（V/V）的10%、MBC分别为占培养液体积（V/V）的20%和40%；山豆根、肉豆蔻提取液对核盘菌的MIC分别为占培养液体积（V/V）的20%和10%，MBC分别为占培养液体积（V/V）的40%和20%。

Abstract:

The inhibition effects of chinese herbal extracts against Alternaria alternate and Sclerotinia sclerotiorum causing decay of postharvest broccoli were studied in this paper. The antibacterial effects of different Chinese herbal extracts were compared by growth rate and filter paper methods. Then the living verification test was used on broccoli. The species of Chinese herbal showing good inhibitory effects were selected and their minimal inhibition concentration (MIC) and minimal bactericidal concentration (MBC) were investigated by dilute with grads. The results showed that different natural plant extracts had different inhibitory effects against the microbes in postharvest broccoli. Subprostrate sophora and myristica fragrans were the better kinds showing inhibitory capability against Alternaria alternata and Sclerotinia sclerotiorum among 15 kinds of natural plant material. The MIC of the extracts of Subprostrate sophora and myristica fragrans for Alternaria alternata were 10% (V/V). And the MBC of Subprostrate sophora and myristica fragrans extracts for Alternaria alternata were 20% and 40%, respectively. For Sclerotinia sclerotiorum, the MIC of subprostrate sophora and myristica fragrans extracts were 20% and 10% (V/V), respectively, and the MBC of subprostrate sophora and myristica fragrans extracts were 40% and 20%, respectively.