黄芪多糖对肿瘤模型小鼠红细胞免疫功能的影响

Effect of Astragalus Polysaccharide on Immune Function of Erythrocyte in Tumor Model Mice

摘要:

本文研究了黄芪多糖对肿瘤模型小鼠红细胞膜结构组分和免疫功能的影响。黄芪多糖对S180小鼠腹腔注射给药7 d，聚丙烯酰胺凝胶电泳测定小鼠红细胞膜带3蛋白及血型糖蛋白A的相对含量，采用试剂盒测定红细胞膜磷脂、胆固醇和唾液酸含量，荧光偏振法测定红细胞膜流动性。应用流式细胞仪测定红细胞CR1数量；显微镜观察肿瘤红细胞花环率(DTER)和C3b受体花环率(RBC-C3bRR)。黄芪多糖可以使S180小鼠红细胞膜磷脂含量升高0.17 mmol/L、胆固醇含量降低0.06 mmol/L；可以显著增加S180小鼠红细胞膜带3蛋白及血型糖蛋白A的相对含量(2.88%、7.40%)和增加0.23 mmol/L红细胞膜唾液酸含量；提高S180小鼠红细胞膜流动性；还可以使S180小鼠红细胞CR1数量增加5.9%，S180小鼠红细胞DTER和RBC-C3bRR增加3.13%和3.26%。黄芪多糖可以改善肿瘤机体红细胞膜组分及结构，从而维持细胞膜的流动性，恢复红细胞的功能；还可以增加肿瘤机体红细胞CR1的数量，增强红细胞CR1天然免疫活性，改善肿瘤机体红细胞免疫功能

Abstract:

The effect of Astragalus polysaccharide on membrane structure and components of erythrocyte were studied and its effect on erythrocyte immune function in tumor model mice was also studied. S180 mice were made as tumor model mice, and administrated with Astragalus polysaccharide by i.p. for 7 d, and then the erythrocytes in mice venous blood were collected. Band 3 and glycophorin A content were determined by SDS-PAGE. Spectrophotometer with test kit was used to measure phospholipids, cholesterol and sialic acid. DPH dye and fluorescence spectrophotometer were used to determine membrane fluidity. Erythrocytes were labeled by rat anti-mouse CR1 monoclonal antibody and FITC-conjugated goat anti-mouse antibody. Flow cytometry was used to determine the number of CR1, and then the DTER and RBC-C3bRR were determined by microscope. The results showed that Astragalus polysaccharide increased phospholipids content by 0.17 mmol/L and decreased cholesterol content by 0.06 mmol/L. In addition, the contents of Band 3, glycophorin A proteins and sialic acid were increased by 2.88%, 7.40% and 0.23 mmol/L, respectively. Erythrocyte membrane fluidity was also improved remarkably. The use of Asparagus polysaccharide also resulted in the increase of CR1 number, DTER and RBC-C3bRR in tumor model mice by 5.9%, 3.13% and 3.26%, respectively. These results indicated that Astragalus polysaccharide can adjust the abnormality of erythrocyte in tumor model mice, improve membrane structure and components, and restore membrane fluidity and function. Asparagus polysaccharide can also improve the erythrocyte immune function by affecting CR1.