荔枝果肉乙酸乙酯粗提物对Th1/Th2细胞平衡的影响

Effect of Ethyl Acetate Extracts from Litchi Fruit on the Th1/Th2 Cell Balance in Mice

摘要:

本研究分析荔枝果肉乙酸乙酯部粗提物对小鼠细胞因子分泌的作用，进而探讨其对Th1/Th2细胞平衡的影响。将30只BALB/C小鼠随机分成对照组（生理盐水）、乙酸乙酯部粗提物低剂量和高剂量组（4.6、9.2 mg/kg?bw?d），连续灌胃7 d，观察各组小鼠的胸腺和脾脏指数变化以及脾细胞增殖情况，用ELISA检测小鼠脾细胞以及血清分泌IFN-γ和IL-4的含量以及IFN-γ/IL-4比值。实验结果表明：乙酸乙酯部粗提物高剂量组小鼠的胸腺指数、脾脏指数显著提高。乙酸乙酯部粗提物低、高剂量都能显著提高小鼠脾细胞和血清IFN-γ分泌水平，同时，高剂量组小鼠脾细胞和血清IL-4的分泌水平也显著提高。低、高剂量的乙酸乙酯部粗提物都能显著提高小鼠脾细胞和血清IFN-γ/IL-4的比值。荔枝果肉乙酸乙酯部粗提物诱导Thl/Th2平衡向Th1方向漂移，可能进而诱导机体的炎症性反应。

Abstract:

The effect of ethyl acetate extracts (EAE) from litchi fruit on cytokine production of mice was analyzed, and then its effect on the Th1/Th2 cells balance was explored. 30 BALB/C mice were divided into the control group (NC), the low dose group (EL, 4.6mg EAE/kg?bw?d), and the high dose group (EH, 9.2mg EAE/kg?bw?d). After 7 d, the index of spleen and thymus gland, and the spleen cells proliferation were detected. The secretion of IFN-γ and IL-4 in spleen cells and serum were determined by ELISA. The results indicated that high-dose EAE could increase the index of spleen and thymus gland. Compared with NC group, IFN-γ secretion IL-4 of spleen cells and serum in EL and EH groups were significantly increased. Higher secretion of spleen cells and serum in EH groups were found compared with NC group. IFN-γ/IL-4 ratio of spleen cells and serum in both EL and EH groups were also significantly improved compared with NC group. EAE from litchi fruit may possess inflammatory response by shifting the Th1/Th2 balance toward Th1 polarization.