微波辅助提取叶下珠中多糖工艺研究

Microwave-assisted Extraction of Polysaccharide from Phyllanthus urinaria

摘要:

本研究利用微波辅助法提取叶下珠多糖，采用苯酚-硫酸法测定叶下珠多糖含量，通过正交实验考察了提取时间、提取功率和固液比（m叶下珠粉/V水）对叶下珠多糖提取率的影响。结果表明，微波提取时间对叶下珠多糖的得率影响最为显著，提取时的功率影响次之，固液比的影响最小，在微波提取时间为40 min、固液比1:20和提取功率400 W时，叶下珠多糖得率最高为7.9%。

Abstract:

In this paper, micro-assisted extraction of polysaccharide from Phyllanthus urinaria was studied. The content of polysaccharide was determined by the method of phenol-sulfuric acid. Three main factors including extraction time, extraction power and ratio of solid to liquid were studied by orthogonal experiments. Results showed that extraction time was the most significant influential factor, followed by extraction power, and ratio of solid to liquid showed the minimum influence. The optimum extraction conditions were: extraction time 40 min, ratio of solid to liquid 1:20 and extraction power 400 W, under which the extraction yield of polysaccharide reached 7.9 wt%.