响应面法优化超高压辅助提取茶多酚的工艺研究

Optimization of Ultra High Pressure -assisted Extraction of Tea-polyphenols by Response Surface Methedology

摘要:

为优化茶多酚的超高压提取工艺，在单因素试验基础上，选择乙醇浓度（X1）保压时间（X2）及作用压力（X3）为自变量，茶多酚提取得率（Y1）为响应值，利用Box-Benhnken 中心组合试验和响应面分析法，研究各自变量交互作用及其对茶多酚提取得率的影响，模拟得到二次多项式回归方程的预测模型，并确定最佳提取工艺条件为乙醇浓度60%、保压时间1.5 min和作用压力300 MPa。在该条件下茶多酚提取得率的预测值为31.07%，验证值为30.87%，误差为2.59%。预测模型的标准回归方程为Y1=776.3561-1.3356X1+123.093X2+163.7406X3-274.2023X1X1-145.2251X2X2。

Abstract:

To obtain the optimum ultra high pressure-assisted extraction technology of tea-polyphenols from low value tea. On the basis of single-factor test, the mathematical regression model is established about the dependent variable(extraction yield of tea-polyphenols ) and independent variables (alcohol concentration, time of maintain pressure and extraction pressure) through Box-Benhnken center composite design and response surface methodology. The results indicated that the optimal extraction conditions were alcohol concentration of 60%, processing time of 1.5 min, extraction pressure of 300 MPa. Under these conditions, the predictive maximum yield of tea-polyphenols from low value tea was 31.07%, the validation value was 30.87%. The relative error between them was 2.59% .The standardized regression equation was Y1=776.3561-1.3356X1+123.093X2+163.7406X3-274.2023X1X1-145.2251X2X2.