响应面法优化加拿大原产地西洋参皂苷的提取工艺研究

Optimization of Extraction Process of Ginsenoside from Panax quinquefolius L. Collected from Canada by Response Surface Methodology

摘要:

优化加拿大原产地西洋参皂苷的最佳提取工艺。采用HPLC-ELSD法，在单因素试验基础上，利用响应面法，以人参皂苷Rb1为响应值，建立数学模型，对提取温度、时间、乙醇浓度和料液比进行优化，确定最佳提取工艺。结果表明：提取温度为90 ℃、乙醇浓度77.4%、提取时间1.88 h、料液比1:25、提取2次的人参皂苷Rb1提取率可达到1.40%，与预测值1.41%相差不大。

Abstract:

The Response Surface Methodology (RSM) was used to optimize the extraction process of ginsenoside from P. quinquefolius L. collected from Canada. Based on one-factor-at-a-time experiments, extract yield of the ginsenoside-Rb1 was used as response for establishing a quadratic regression model. Temperature and time of extraction, ethanol concentration and liquid-to-solid ratio were selected as the factors for RSM design. The results showed that the best extraction conditions were as follows: extraction temperature 90 ℃, extraction time 1.88 h, ethanol concentration 77.4%, solid-liquid ratio 1:25, and extracted times twice, under which the predicted yield of ginsenoside-Rb1 with the optimized procedure was up to 1.40%and practical yield was 1.41%.