菌株SFZ-37产鼠李糖脂分批式发酵动力学的初步研究

Kinetics Study of Batch Fermentation of Rhamnolipid by Pseudomonas aeruginosa SFZ-37

摘要:

在5 L磁力搅拌发酵罐上，对铜绿假单胞菌（Pseudomonas aeruginosa）菌株SFZ-37生产鼠李糖脂进行分批发酵，并对发酵过程进行监测，pH值在发酵过程中基本维持稳定，发酵结束时，菌体生物量可达4.41 g/L，鼠李糖脂产量达到11.61 g/L，底物甘油残量达到11.69 g/L。根据实验数据构建了菌体生长、产物生成及底物消耗的动力学模型，并采用OriginPro 8.0软件对其进行了非线性拟合。结果表明，建立的动力学模型可以较好的反映菌株分批发酵过程中菌体生长、产物生成和底物消耗三者之间的关系。

Abstract:

To explore rhamnolipid production by Pseudomonas aeruginosa SFZ-37, batch fermentation was conducted in a 5-L fermentor and the fermentation process was monitored. During the batch fermentation, the pH was almost constant. At the end of batch fermentation, the dry cell weight, rhamnolipid production and residual glycerol could reach 4.41 g/L, 11.61 and 11.69 g/L, respectively. Based on the experimental data of batch fermentation, the kinetic models of cell growth, product formation and substrate consumption were established. The non-line fitting was employed to model the kinetics of fermentation by software OriginPro 8.0. The kinetic model could be used to describe the relationship of cell growth, product formation and substrate consumption during the process of batch fermentation.