产细菌素嗜盐四联球菌的筛选及鉴定

Identification and Screening of Bacteriocin-producing Tetragenococcus halophilus

摘要:

从传统露天酿造工艺的酱油中分离出12株乳球菌，对其进行形态、生理生化特性研究及16S rDNA序列分析，鉴定为嗜盐四联球菌。在排除酸作用后，WZ-3菌株发酵液对革兰氏阳性和革兰氏阴性细菌具有较好的抑菌活性，经胃蛋白酶、胰蛋白酶处理后，发酵液抑菌活性略有下降。因此初步认为该菌株为产细菌素的嗜盐四联球菌。

Abstract:

Twelve strains lactococcus was isolated from the traditional Chinese soy. the physiological and biochemical characteristics and the 16SrDNA sequence of the screened strain were then analyzed, based on which the strain was identified as Tetragenococcus halophilus. The supernatant of WZ-3 strain eliminate the organic acids exhibited strong antibacterial activity against the gram-positive and gram-negative bacteria. The antibacterial activity decreased slightly after treatment with pepsin and trypsin, therefore, we think the strain was a bacteriocin-producing Tetragenococcus halophilus.