两种微藻浓缩液保藏效果的比较

Evaluation of the Preservation Methods for Two Microalgal Concentrates

摘要:

本试验研究了不同冷冻保护剂和降温方式处理下，眼点拟微球藻（Nannochloropsis oculata）浓缩液（2×109 cells/mL）在4 ℃和-18 ℃下保藏3个月，以及钝顶螺旋藻（Spirulina platensis）浓缩液（7%干重）在-18 ℃下保藏3个月的效果。结果表明，采用-18 ℃速冻、不添加保护剂，保藏3个月后眼点拟微球藻的相对细胞活性最高，达到新鲜细胞对照的48.17%，显著高于其他处理组(P<0.05)；同样条件下，眼点拟微球藻浓缩液的蛋白和EPA损失率分别为3.42%和2.73%，钝顶螺旋藻浓缩液的蛋白和总脂肪酸损失率分别为4.17%和19.33%，综合比较各处理，该方法是这两种微藻浓缩液低温保藏的优选方法。

Abstract:

Various cryoprotectants and cooling methods were adopted for 3-month preservation of the concentrates of Nannochloropsis oculata (2×109 cells/mL,at 4 ℃ and -18 ℃) and Spirulina platensis (7% dry weight at -18 ℃). The results showed that, the highest relative cell activity of N. oculata concentrates preserved for 3 months reached 48.17% of that control (fresh cultured cells) by fast freezing at -18 ℃ without cryoprotectant, which higher than other treatments significantly (P<0.05). Under the same conditions, the loss of protein and EPA in the concentrates of N. oculata had were 3.42% and 2.73%, respectively,The lose of the protein and total fat acids in the concentrates of S. platensis were 4.17% and 19.33%, respectively. Based on the systematic comparison, the method of fast freezing at -18 ℃ without cryoprotectant was determined as the favorable for preservation of the concentrates of N. oculata and S. platensis at low temperature.