呋喃唑酮代谢物抗体的制备与筛查

The preparation and screening of antibody of Furazolidone Metabolite

摘要:

利用呋喃唑酮的4种代谢物合成免疫原以及包被原，用4种免疫原各免疫两只新西兰大白兔，得到8份抗血清，再纯化抗血清。采用间接ELISA方法，筛选出OD值明显高于阴性样本的血清用于以后的实验，同时对抗体的效价进行测定。实验检测结果表明，当用包被原A和包被原B包被酶标板时，7号和8号抗血清对该包被抗原有很好的亲和性，呋喃唑酮代谢物衍生物对抗体有很好的抑制。通过效价检测，7号和8号抗血清均具有高效价、高特异性的特点，其中8号抗血清的效价更高，亲和力更强。再通过进一步对两种包被原和两份抗血清进行筛查，选择B包被原和8号兔子的抗体清用于ELISA法的建立。

Abstract:

Using of 4 Furazolidone Metabolite Residues, immunogen and coating antigen were synthesized. Then, 4 immunogen were used to immunize 2 New Zealand rabbits. 8 antiserums were obtained and purified. Using indirect ELISA method, the OD value which was higher than that of negative samples of serum were filtered out and used in subsequent experiments, and then antibody titer were determined. The results implied when coating antigen A and B coated microtiter plates. No.7 and No.8 antiserums had a good affinity on the coating antigen. The derivatives of Furazolidone Metabolite had a good effect on antibody inhibition. Through the determination of antibody titer, No.7 and No.8 antiserums both had high titer and high specificity features, of which NO.8 antiserum provided higher antibody titer and higher affinity. Through further screening of 2 coating antigen and 2 antiserums, coating antigen B and No.8 antiserum were chosed for ELISE method.