新琼寡糖作为免疫增强剂在鲍鱼养殖中的应用

Application of Neoagaro-oligosaccharides as Immunostimulants in Abalone Production

摘要:

采用一种活性新琼寡糖作为免疫增强剂，通过在饲料中添加不同含量的新琼寡糖(分别为饲料重量的0.1%，0.5%，1%，1.5%和3%)，以血清溶菌酶活力、血清补体活性及白细胞总数和白细胞吞噬率为判断依据，确定新琼寡糖对于鲍鱼非特异性免疫力的影响；同时用嗜水气单胞菌（Aeromonas hydrophila）对各组鲍鱼进行细菌感染试验，测定鲍鱼存活率，以确定新琼寡糖对鲍鱼抗病力的影响。结果表明：投喂新琼寡糖组的鲍鱼的血清溶菌酶活力、血清补体活性及血液白细胞总数和吞噬率均明显高于对照组，且新琼寡糖的最佳投喂量为饲料重量的0.5%~1%。细菌感染试验结果显示，在细菌感染72 h后，对照组鲍鱼的存活率最低，仅为42%，而新琼寡糖投喂组鲍鱼的存活率均达到58%以上，且最高可达到79%。

Abstract:

In this study, the activity of neoagaro-oligosaccharides as a new immunostimulant was studied by adding different dosages of Neoagaro-oligosaccharides in the feed (0.1%, 0.5%, 1%, 1.5% and 3%, respectively). Then, determination of serum complement activity, lysozyme activity, phagocytic activity and the total number of leucocytes was carried out to decide the impact of neoagaro-oligosaccharides on abalone non-specific immunity. Besides, the abalone were injected peritoneally with Aeromonas hydrophila after fed 3 months for determine the effect of neoagaro-oligosaccharides on the disease resistance. The results showed that the serum complement activity, lysozyme activity, phagocytic activity and the total number of leucocytes of the groups of adding neoagaro-oligosaccharides in the feed were significantly higher than that of the control group. The best dosage of the Neoagaro-oligosaccharides in the feed was between 0.5% and 1%. The results of the bacterial infection test showed that the survival rate of neoagaro-oligosaccharides feeding group was significantly higher than that of the control group.