纤维素降解菌株的筛选及其产酶条件优化

Studies on Screening of Methane Fermentation Cellulose degrading Bacteria and its Optimization of Culture Conditions

摘要:

为了寻求快速有效降解沼气发酵有机质中的高分子化合物，本实验从腐殖质土壤中筛选到一株高效降解纤维素菌株，在以羧甲基纤维素钠为唯一碳源的液体培养基中培养，所产生的纤维素酶对玉米芯和滤纸均表现出较强降解能力。其次做了对菌株培养条件优化的实验，结果表明，菌株的最佳降解纤维素条件为反应温度30 ℃、发酵液接种量为1%、0.75%羧甲基纤维素钠为碳源、1.5%胰蛋白胨为氮源，优化菌株培养条件后，纤维素酶活力增加了2.8倍。

Abstract:

In order to be effective, rapid degradation of organic polymer compound biogas fermentation, from soil degradation to a plant screening high efficiency degradation cellulose strain. In sodium carboxyl methyl cellulose as the only carbon source of liquid medium training strains, the cellulose enzyme produced for several and filter paper are show have better degradation ability. Next to the optimization of the strains of the culture condition experiment results show that the best cellulolytic strains of the conditions for the reaction temperature 30℃, fermented liquid 1% inoculated quantity, carbon source sodium carboxyl methyl cellulose 0.75%, had 1.5% nitrogen pancreatic specially designed, and so on. Optimization strains after the culture condition, cellulose enzyme activity has increased 2.8times.