凝胶色谱净化/气相色谱法测定鳗鱼中多种有机磷农药残留量

Determination of Organophosphorus Pesticide Multi-residues in Eel by Gas Chromatography with Gel Permeation Chromatography Clean-up

摘要:

建立了凝胶色谱技术（GPC）净化，用气相色谱法测定水产品鳗鱼中11种有机磷农药残留量的方法。样品用乙酸乙酯/环己烷溶液（1+1，V/V）超声提取，GPC净化，收集所需流分,浓缩近干后用乙酸乙酯溶解定容，气相色谱分析。方法检测限（LOQ）为0.01~0.02 mg/kg，方法回收率为76.3%~106.8%，相对标准偏差（RSD）为2.3%~15.8%。结果表明，该方法操作简便快速、灵敏度高，符合实际应用需要。

Abstract:

A method was developed for determining of 11 organophosphorus pesticide residues in eel by Gas Chromatography (GC) with Gel Permeation Chromatography (GPC) clean-up. The samples were extracted using the solution of ethyl acetate and cyclohexane (1+1, V/V) with ultrasonic machine and then cleaned up by GPC. The elution needed was ccollected and concentrated to nearly dryness. The residue was dissolved with ethyl acetate for determination by GC-FPD. The limit of quantification (LOQ) was 0.01~0.02 mg/kg. The recovery ranges were 76.3%~106.8% when the added levels were from 0.02 mg/kg to 1.0 mg/kg. The relative standard deviations (RSD) were 2.6%~13.8%. The method was simple, sensitive and suitable for the analysis of pesticide residues.