天蚕素抗菌肽发酵培养基优化

Optimization of the Fermentation Medium of Cecropins Production

摘要:

选用抗菌肽生产菌KJ02为出发菌，对其发酵培养基进行了研究并使用正交设计优化了发酵培养基。优化后的培养基：葡萄糖3%，蛋白胨1%，酵母膏2%，NaCl 0.5%。同时在500 L中式水平上对该培养基的效果进行了试验，发酵液杀菌效价为13651 IU/mL。

Abstract:

The fermentation medium of a cecropin-produntion strain KJ02 were studied and optimized by orthogonal test. The optimal components for fermentation medium were glucose 3%, peptone 1%，yeast extract2% and NaCl 0.5%. With the optimal medium, the cecropin efficiency in 500 L fermentator was up to 13651 IU/mL.