WprA基因在Bacillus subtilis WB800中的克隆表达

Clonging and Expression of a WprA gene in Bacillus subtilis WB800

摘要:

对源自Bacillus subtilis 168的具有纤溶活性的基因序列（WprA）进行克隆，然后将WprA基因克隆到大肠杆菌-枯草杆菌穿梭载体 pBE3中，构建表达载体pBE-WprA，将重组载体转化到Bacillus subtilis WB800中，实现了WprA基因在Bacillus subtilis WB800中的表达。结果表明，WprA基因在Bacillus subtilis WB800中的对数生长期和平衡期均获得了表达，且产物被分泌到胞外。

Abstract:

A fibrinolytic enzyme gene (WprA) was cloned from Bacillus subtilis 168. To efficiently express WprA in Bacillus subtilis WB800, WprA gene was inserted into pBE3 to yield a nove vector pBE-WprA. Then the vector pBE-WprA was transformed and expressed in Bacillus subtilis WB800. Results showed WprA gene was efficiently expressed during the exponential and stationary growth stages, and WprA was secreted into the medium.