超高效液相色谱法测定保健食品中的多种水溶性维生素

Determination of Water-soluble Vitamins in Health Foods by UPLC

摘要:

本文应用超高效液相色谱法，Acquity UPLC HSS T3 (2.1×100 mm×1.8 μm)柱，0.1%三氟乙酸-乙腈流动相，梯度洗脱，紫外和荧光串联检测器，可同时测定保健食品中6种水溶性维生素，其中包括Vc、B3、B1、B6、B11、B2等，该法线性范围宽、呈直线回归、相关性好、检测限低、分析速度快，其测定结果与国标法相吻合。

Abstract:

Water-soluble vitamins were determiend by Ultra-performance liquid chromatography (UPLC) equipping ultraviolet detector and fluorescence detector in series. Samples were separated by a Acquity UPLC HSS T3 (2.1×100 mm×1.8 μm) column using 0.1% trifluoroacetic acid-acetonitrile as mobile phase in gradient elution mode. Six water-soluble vitamin components in health foods were simultaneously determined, including ascorbic acid (Vc), nicotinamide (B3), thiamine (B1), pyridoxine (B6), folic acid (B11), riboflavin (B2), etc. This method was enabled to achieve wide linear range, well correlation, low detection limit and fast analysis. The results were in accordance with those wtih GB methods.