氧化镍-脂肪酶修饰丝网印刷电极测定二油酸甘油酯

Detection of Dioleglyceride Using NiO-lipase Modified Screen-printed Electrode

摘要:

利用电化学氧化沉积纳米氧化镍(NiO)于丝网印刷电极(SPE)，使用壳聚糖包埋法将脂肪酶固定在NiO-SPE上构建一种新的脂肪酶生物传感器用于测定二油酸甘油酯。以铁氰化钾作为电子媒介体，在1% Triton-X100，PBS（0.1 mol/L, pH 7.0）体系中，用循环伏安法（CV）表征了二油酸甘油酯在Lipase-NiO-SPE上的伏安行为，并用CV检测二油酸甘油酯。在0.04~180 μmol/L范围内呈现良好线性关系(R=0.993)，检出限为0.01 μmol/L。该方法简单、快速、准备、稳定，可用于二油酸甘油酯含量的检测。

Abstract:

A new enzyme sensor for determination of dioleglyceride was constructed by nano-nickle oxide modified through electro- chemical oxidation sedimentary reaction on the screen-printied electrode (SPE) and lipase immobilized by chitosan. Using ferricyanide as the electron mediator, , the CV behavior of dioleglyceride on lipase-NiO-SPE were evalutaed in the solution of 1% Triton-X100 and PBS buffer (0.1 mol/L, pH 7.0). A good linear relationship was obtained between dioleglyceride ranging in 0.04~180 μmol/L with cyclic voltammetry (R =0.993) and detection limit of 0.01 μmol/L. This method was simple, rapid, accurate, and steady for the determination of dioleglyceride.