桔青霉高产核酸酶P1的固态发酵条件研究

Optimization of the Nuclease P1 Production Conditions by Penicillium citrinum in Solid State Fermentation

摘要:

以本实验室从土壤中分离筛选到的一株高产核酸酶P1的桔青霉GX-K为生产菌种，研究了该菌固体发酵产核酸酶P1的发酵条件。结果表明其最佳产酶条件为：以麸皮作为固态发酵基料，添加4%的蔗糖，料水比为1:0.9，30 ℃培养72 h。该条件下核酸酶P1产量达5783.41 U/g干基。

Abstract:

The conditions for solid fermentation of a strain of Pecillium citrinum GX-K with high yield of nuclease P1 were studied. Wheat bran was US as the substrate of solid fermentation and the other optimum fermentation conditions were: 4% of sucrose, substrate-water ratio 1:0.9, fermentation temperature 30 oC and fermentation time 72 h. The activity of nuclease P1 under the optimum fermentation conditions reached 5783.41 U/g.