诃子活性成分的抗氧化活性及其结构鉴定

Free-Radical-Scavenging Activity of Various Components in Terminalia Chebula Ritz

摘要:

本实验中制备了诃子不同溶剂粗提物及部分纯化物，并考察了它们的清除自由基活性。1.1-二苯基-2-苦肼基（DPPH?）清除实验结果表明，所有粗提物均具有很强的活性，其顺序为：甲醇＞乙酸乙酯＞丙酮＞乙醚＞石油醚。其中甲醇粗提物（1 mg/mL）活性略高于等浓度的TBHQ；色谱纯样a比其原粗提取物（乙酸乙酯）的抑制率提高最多，为其1.6倍，而甲醇粗提物纯化样c的绝对活性最高，约为同浓度TBHQ（1 mg/mL）的1.2倍，且略高于茶多酚。它们的化学结构经显色反应、UV-Vis和H1 NMR检测推定：a为诃黎勒酸，b为棓酸衍生物，是诃子单宁的小水解碎片，而c是诃黎勒酸的变体。

Abstract:

In this experiment the free radical-scavenging activity of various components from terminalia chebula ritz was studied. The results showed that all extracts of terminalia chebula ritz stems had strong DPPH? scavenging effects. The activity-decreased sequence of the extracts by different extracting regents was: methanol>ethyl acetate>acetone>ether>petro-ether. The methanol extract (1mg/mL) had better activity than that of TBHQ. The activities of the component A was 1.6 folds higher than the original. The chromatographically purified component C showed the strongest activity among all samples. Its activity was a little stronger than that of tea polyphenol, as 1.2 folds higher than that of TBHQ. By color test, UV-Vis and H1 NMR analysis, all of the purified components were confirmed to be the derivatives of myrobalani-tannin or their hydrolyzed segments.