婴儿粪便中乳酸菌的分离鉴定及其对小鼠腹泻的治疗作用

Isolation and Identification of the Lactic Acid Bacteria from Infant Faeces and their Therapeutical Effect on Diarrhea of Mice

摘要:

从健康婴儿新鲜粪便中分离纯化出6株产酸能力强的革兰氏阳性菌，并对其进行了体外抑菌试验研究。筛选出对致腹泻性大肠杆菌（O78、O88、O149）均具有良好抑制作用的3个菌株，经过生理生化试验以及16S rRNA分析的鉴定，表明3株均为干酪乳杆菌(Lactobacillus casei)。然后，挑选一株对致病性大肠杆菌抑菌性最强的菌株进行小鼠和鸡的攻毒试验，其结果表明该菌株安全无毒，且能适当提高动物的料肉比，起到一定的预防和治疗腹泻的作用。

Abstract:

Six Gram-positive bacteria with strong acidogenicity were separated and purified from fresh feces of healthy babies, and their bacteriostasis in vitro were tested. Three strains with high inhibition effect on diarrhoeic Escherichia coli (O78, O88 and O149) were screened. The results of biochemical identification and 16S rRNA comparison analysis showed that all the three screened strains were Lactobacillus casei. The challenged test in mice and chickens by one strain with strongest inhibition on pathogenic E. coli showed that the strain was safe, nontoxic and appropriately improved the animal feed conversion rate, which can used for prevention and treatment of diarrhea effect.