虎杖中白藜芦醇生物转化菌的筛选及鉴定

Screening and Identification of Strain for Resveratrol Biotransform from Polygonum cuspidatum

摘要:

白藜芦醇具有多种生物学活性，具有很高的应用价值，白藜芦醇的前体物质白藜芦醇苷在虎杖中含量较高，可通过微生物转化成白藜芦醇。论文采用添加有白藜芦醇苷的固体培养基，从虎杖内筛选能将白藜芦醇苷转化成白藜芦醇的内生菌，然后对筛选到的菌种进行鉴定。通过菌种形态学、显微镜观察和18S rDNA序列分析等方法，鉴定结果显示该菌种为卡门培尔青霉(Penicillium camemberti)。

Abstract:

Resveratrol is a grape polyphenol with a variety of bioactivities.The precursor substances of resveratrol, polydatin, have high content in plants, such as Polygonum cuspidatum. In this paper, screening medium with polydatin was used to screen a strain that can convert polydatin into resveratrol from Polygonum cuspidatum. Then the screened strain was identified by morphology and 18S rDNA sequence analysis methods. The identified results showed that the strain was Penicillium camembert.