云南野生香薷油对子代小鼠学习记忆功能的影响

Effect of Yunnan wild Elsholtzia Oil on Learning and Memory in Offspring Mice

摘要:

本研究旨在探讨云南野生香薷油对子代小鼠学习记忆功能的影响。将昆明种小鼠从受孕第1 d开始分为空白对照、阳性对照、低、中、高剂量5个组，用云南野生香薷油灌胃至断乳，断乳♂小鼠按孕鼠剂量灌胃至出生49 d后做Morris水迷宫实验，之后测定小鼠脑组织神经递质和肝、脑组织的脂肪酸。结果表明，与空白对照组比较，定位航行实验的第5 d各组均有统计学意义（P<0.01）。空间探索实验的阳性对照组有统计学意义（P<0.05）。脑神经递质TPro、NO、AchE：阳性、中、高剂量组均有统计学意义（P<0.05）。肝脂肪酸结果中阳性和高剂量组的ALA有统计学意义（P<0.05）；脑组织脂肪酸结果：高剂量组的油酸和ALA有统计学意义（P<0.05）。可见，富含?-亚麻酸的香薷油具有部分改善子代小鼠学习记忆的功能，其作用可能与喂养此香薷油使脑组织的TPro升高，NO、AchE降低；肝和脑组织的?-亚麻酸升高有关。

Abstract:

This study aimed to investigate the effect of Yunnan wild elsholtzia oil on learning and memory function of mouse offspring . From the first day of pregnancy, Kunming mice were divided into five groups: blank control group, positive control group, low-dose, medium-dose and high-dose groups and were intragastrically administrated with the elsholtzia oil gavage till being weaned. According to intragastic administration dose for the pregnant rats, the weanedmale mouse was intragastically administrated till 49 days after birth and then tested for the Morris water maze test. The fatty acid contents of the liver and brain organization were detected. Compared to the blank control group, navigation test showed that each group were statistically significant (p<0.01) in the 5 th day. Space exploration experiment showed that only the positive control group was statistically significant (p<0.05). By analysis of TPro, NO, AchE , it was found that he positive control group, medium-dose and high-dose groups were statistically significant (P<0.05). The contents of the fatty acids in liver of the positive control group and the high-dose group were statistically significant (p<0.05). Contents of oleic acid and ALA in high-dose group were statistically significant (p<0.05) in brain tissue. ALA-riched eElsholtzia oil can partly enhance the learning and memory in mouse offspring mouse., due to the hihger TPro and ALA contents but lower NO and AchE contents by feeding ALA-riched elsholtzia oil.