高效液相色谱法测定酱油中的5-羟甲基糠醛

Determination of 5-Hydroxymethylfurfural in Soy by HPLC

摘要:

采用高效液相色谱法测定酱油中5-羟甲基糠醛（5-HMF）的含量。样品经乙酸乙酯提取后，用C18色谱柱分离，以甲醇-水（体积比为5:95）为流动相，在UV 284 nm波长条件下进行检测。该方法快速、准确，在0.5~20 mg/L范围内线性相关系数为r2=0.9992，平均回收率为82.4%~ 86.9%，相对标准偏差为3.2%~5.1%。方法的最低检测浓度为0.1 mg/L。

Abstract:

A new method for the determination of 5-hydroxymethylfurfural by high performance liquid chromatography in soy was developed. After extracted by ethyl acetate, the samples were analyzed on a C18 column at 284 nm using a mixture of methanol and water (5:95, v/v) as the mobile phase . Within the linear range of 0.5~20.0 mg/L, the method was fast and accurate with the average recoveries rate and relative standard deviation of 82.4%~86.9% and 3.2%~5.1% , respectively. The minimum determination limit was 0.1 mg/L.