苯酚-硫酸比色法测定毛竹叶多糖的研究

Determination of Polysaccharides in Phyllostachys pubescens Leaves by Phenol-vitriolic Colorimetry

摘要:

对苯酚—硫酸比色法测定毛竹竹叶中的多糖含量进行了研究。结果表明此法灵敏度高，重复性好，多糖最佳检测波长为490 nm，在浓度20~100 μg/mL范围内线性相关系数R2=0.9993，重现性标准差为0.0055，回收率99.22%。

Abstract:

The content of total polysaccharides in Phyllostachys pubescens leaves by phenol- vitriolic colorimetry were studied. Results showed that this method was credible and reproducible and the linear concentration range was within 20~100 μg/mL (R2=0.9993) at the best detection wavelength of 490 nm, recurrence standard deviation was 0.0055 and the rate of recycle was 99.22%.