杨桃中多糖成分的纯化与鉴定

Purification and Identification of Polysaccharides from Averrhoa caramhola L.

摘要:

本文对采用水煮乙醇沉淀法粗提出的杨桃多糖成分进行纯化与鉴定。首先将粗多糖用复合酶法去蛋白，然后用凝胶过滤柱进行层析纯化，最后通过紫外、红外扫描对纯化后的多糖进行纯度和结构鉴定及分子量测定。初步鉴定的结果表明，杨桃多糖的平均分子量为4.373×104，组分中含有乙酰氨基、硫酸基、吡喃环结构、β-D-甘露吡喃糖环、α-D-半乳吡喃糖结构和α-D-甘露吡喃糖结构，实验结果表明，杨桃多糖中含有β-D-甘露吡喃糖、α-D-半乳吡喃糖、α-D-甘露吡喃糖3种成分。

Abstract:

Crude Averrhoa caramhola L. polysaccharides, obtained by extraction with hot water and precipitation with ethyl alcohol, were purified by gel filtration chromatography after removing the free proteins and then identified by ultraviolet spectrum scanning and IR spectroscope. The results showed that the average molecular weight of the Averrhoa caramhola L. polysaccharides was 4.373×104. And the polysaccharides consisted of β-D-mannose pyranose, α-D-Galactopyranose and α-D-mannose pyranose.